Clare M G, Blain E, Taylor J H
Eur J Cancer Clin Oncol. 1982 Jun;18(6):533-44. doi: 10.1016/0277-5379(82)90222-x.
Lymphocytes from peripheral blood of either 'normal' donors or patients with cancer were stimulated to divide in culture medium containing 5-bromo-2'-deoxyuridine. During 74 hr of incubation, the cells were exposed to single agents or to permutations of two combinations of chemotherapeutic drugs, namely MOPP or cyclophosphamide and methotrexate. Only mustine and cyclophosphamide (activated and not activated) increased the frequency of sister chromatid exchanges (SCE). Neither vincristine, procarbazine or prednisolone with mustine, nor methotrexate with cyclophosphamide altered the number of SCEs expected from the use of mustine or cyclophosphamide alone. There was no difference in the response of cells from cancer patients and 'normal' subjects to the drugs. If the drugs of these two regimens do interact with one another to enhance the amount of subcellular damage, this is not manifested by changes in the number of SCEs in human lymphocytes in vitro.
来自“正常”供体或癌症患者外周血的淋巴细胞，在含有5-溴-2'-脱氧尿苷的培养基中被刺激分裂。在74小时的孵育过程中，细胞暴露于单一药物或两种化疗药物组合的排列组合中，即MOPP或环磷酰胺与甲氨蝶呤。只有氮芥和环磷酰胺（活化的和未活化的）增加了姐妹染色单体交换（SCE）的频率。长春新碱、丙卡巴肼或泼尼松龙与氮芥联合使用，以及甲氨蝶呤与环磷酰胺联合使用，均未改变单独使用氮芥或环磷酰胺时预期的SCE数量。癌症患者和“正常”受试者的细胞对药物的反应没有差异。如果这两种治疗方案中的药物确实相互作用以增加亚细胞损伤的量，那么在体外人淋巴细胞中SCE数量的变化并未体现这一点。
