Ohtsuru M, Ishii Y, Takai S, Kosaki G
Gan. 1982 Jun;73(3):433-8.
Sister chromatid exchanges (SCEs) were investigated in lymphocytes from an adult patient treated with cyclophosphamide (CTX) and vincristine (VCR) for non-Hodgkin's lymphoma. The treatment consisted of iv injection of CTX (1 g) followed immediately by injection of VCR (2 mg). The patient was treated six times at weekly intervals, except for two irregular intervals of 35 and 48 days, respectively, as the second and the fourth intervals. Before the initiation of the therapy, the SCE frequency in the patient was 9.9 +/- 3.5 per cell, which is not significantly different from those of healthy controls and other cancer patients not given chemotherapy. After the first injection, the SCE frequency increased rapidly with time, reaching a peak of 33.4 +/- 4.6 per cell in 24 hr, and then gradually decreased, returning to a nearly normal level of 12.5 +/- 4.2 per cell on the 7th day. Similar cycles of rapid increase and slow decrease in the SCE frequency were observed after each of the subsequent five injections, resulting in a gradual rise in the basal and the maximal levels of the SCE values. Oral administration of CTX (150 mg/day) for about a month during the second interval slightly increased the SCE value from 16.1 +/- 3.1 to 22.4 +/- 6.2 but injections of vincristine alone at a dose of 2 mg twice during the fourth 45-day interval did not affect the gradually decreasing trend in the SCE frequency. In the 5th month after the termination of the therapy, the SCE value returned to the untreated level.
对一名成年非霍奇金淋巴瘤患者的淋巴细胞进行了姐妹染色单体交换（SCE）研究，该患者接受了环磷酰胺（CTX）和长春新碱（VCR）治疗。治疗方案为静脉注射CTX（1g），随后立即注射VCR（2mg）。患者每周治疗一次，共治疗六次，但第二次和第四次间隔分别为35天和48天，间隔不规律。在开始治疗前，患者的SCE频率为每细胞9.9±3.5次，与健康对照者和未接受化疗的其他癌症患者相比无显著差异。首次注射后，SCE频率随时间迅速增加，在24小时内达到每细胞33.4±4.6次的峰值，然后逐渐下降，在第7天恢复到每细胞12.5±4.2次的接近正常水平。在随后的五次注射后，每次都观察到SCE频率快速增加和缓慢下降的类似周期，导致SCE值的基础水平和最高水平逐渐上升。在第二个间隔期间口服CTX（150mg/天）约一个月，使SCE值从16.1±3.1略微增加到22.4±6.2，但在第四个45天间隔期间单独两次注射2mg长春新碱并未影响SCE频率逐渐下降的趋势。在治疗结束后的第5个月，SCE值恢复到未治疗水平。
