通过亲和色谱法分离抗葡萄球菌肠毒素A和E的特异性抗体及共同抗体。
Isolation of specific and common antibodies to staphylococcal enterotoxins A and E by affinity chromatography.
作者信息
Lee A C, Robbins R N, Bergdoll M S
出版信息
Infect Immun. 1978 Aug;21(2):387-91. doi: 10.1128/iai.21.2.387-391.1978.
Abstract
Specific and common antibodies to staphylococcal enterotoxins A and E (SEA and SEE) were isolated from anti-SEA and anti-SEE antisera by affinity chromatography. Anti-SEA and anti-SEE antisera were passed through a column with the cross-reacting enterotoxin coupled to a CNBr-activated Sepharose 4B matrix. Specifically bound common antibodies were eluted with NaSCN. The isolated specific antibodies reacted only with the homologous enterotoxin, whereas the common antibodies gave a reaction of identify with both enterotoxins in double gel diffusion plates. The common antibodies had a higher titer against SEE than against SEA. The significance of the isolation of antibodies common to two separate protein molecules is discussed.
摘要
通过亲和层析从抗葡萄球菌肠毒素A和E（SEA和SEE）抗血清中分离出针对SEA和SEE的特异性及共同抗体。抗SEA和抗SEE抗血清通过与交联反应肠毒素偶联到溴化氰活化的琼脂糖4B基质上的柱。特异性结合的共同抗体用硫氰酸钠洗脱。分离出的特异性抗体仅与同源肠毒素反应，而共同抗体在双凝胶扩散板中与两种肠毒素均呈现同一性反应。共同抗体对SEE的效价比SEA高。讨论了分离两个不同蛋白质分子共有的抗体的意义。
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