Corradin G, Etlinger H M, Chiller J M
J Immunol. 1977 Sep;119(3):1048-53.
An in vitro assay which measures antigen-induced proliferation of primed murine lymph node cells is described. The response is mediated by T eclls since it can be obtained by using nylon wool-passed lymphocytes (less than 1% Ig+ cells) and it can be abolished by treatment with anti-Thy 1.2 and C. Furthermore, LN cells from nu/nu mice injected with antigen do not demonstrate antigen-induced proliferation in contrast to the response observed in euthymic littermate controls. Other relevant parameters of this proliferative assay include the observations that the response is highly antigen specific, can be seen as early as 4 days and as late as 60 days after in vivo priming, is restricted to the use of certain sets of LN when animals are injected subcutaneously at the base of the tail, and can be seen with LN cells from mice primed with antigen in either CFA or ICFA. The ease of the assay coupled with its specificity and quantitative dimensions provides a direct and simple method to evaluate processes involved in antigen-induced murine T lymphocyte activation.
本文描述了一种体外检测方法，该方法用于测定抗原诱导的经致敏的小鼠淋巴结细胞的增殖。该反应由T细胞介导，因为使用尼龙毛柱过滤的淋巴细胞（Ig⁺细胞少于1%）可获得此反应，并且用抗Thy 1.2和补体处理可消除该反应。此外，与在正常胸腺同窝对照中观察到的反应相反，注射抗原的裸鼠的淋巴结细胞未表现出抗原诱导的增殖。这种增殖检测的其他相关参数包括：该反应具有高度抗原特异性，在体内致敏后最早4天、最晚60天均可观察到，当动物在尾根部皮下注射时，仅限于使用某些组别的淋巴结，并且用完全弗氏佐剂（CFA）或不完全弗氏佐剂（ICFA）致敏的小鼠的淋巴结细胞均可观察到该反应。该检测方法简便，兼具特异性和定量特性，为评估抗原诱导的小鼠T淋巴细胞活化所涉及的过程提供了一种直接且简单的方法。
