Stanisic T, Sadlowski R, Lee C, Grayhack J T
Invest Urol. 1978 Jul;16(1):19-22.
Sprague Dawley male rats were injected subcutaneously with either actinomycin D or cycloheximide in saline immediately after castration. Control animals received saline only. Treatment was repeated daily for 4 subsequent days, and 24 hr after the last injection rats were sacrificed by decapitation. Ventral prostates were dissected and weighed. Actinomycin D and cycloheximide administration significantly reduced the rate of prostatic weight loss in castrated rats; serum testosterone levels in the control and drug treated animals were comparable. Histologic studies of the prostates indicated that drug treated animals had more active epithelial cells than saline injected controls. These results indicate that the rate of prostatic regression induced by castration can be modified by drugs such as actinomycin D and cycloheximide.
在去势后，立即给雄性Sprague Dawley大鼠皮下注射生理盐水溶解的放线菌素D或环己酰亚胺。对照动物仅接受生理盐水。在随后的4天里每天重复给药，最后一次注射后24小时，通过断头处死大鼠。解剖并称重腹侧前列腺。给予放线菌素D和环己酰亚胺可显著降低去势大鼠前列腺重量减轻的速率；对照动物和药物处理动物的血清睾酮水平相当。前列腺的组织学研究表明，药物处理的动物比注射生理盐水的对照动物具有更活跃的上皮细胞。这些结果表明，去势诱导的前列腺退化速率可被放线菌素D和环己酰亚胺等药物改变。
