Suppr超能文献

通过直接去除对该抗原无特异性的B细胞来富集抗原特异性B淋巴细胞。

Enrichment of antigen-specific B lymphocytes by the direct removal of B cells not bearing specificity for the antigen.

作者信息

Walker S M, Meinke G C, Weigle W O

出版信息

J Exp Med. 1977 Aug 1;146(2):445-56. doi: 10.1084/jem.146.2.445.

DOI:10.1084/jem.146.2.445
PMID:69002
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2180762/
Abstract

Antigen-specific B cells (ASC) were purified from other B cells by prior incubation with specific antigen followed by rosetting with erythrocytes conjugated with anti-mouse Ig and sedimenting on Ficoll-Isopaque. This procedure allowed the removal of most of the B cells, while those speicifc for the antigen used in incubation were retained. Relative to the B-cell content, ASC were enriched 64- to 132-fold. The method is highly specific in that B cells primed to two different antigens, turkey gamma globulin and sheep erythrocytes, could be separated from each other. The advantages of this indirect purification procedure over purification procedures which obtain ASC directly are the simplicity of obtaining the ASC and the ability of the ASC of respond to antigen without the addition of other cells.

摘要

抗原特异性B细胞（ASC）通过以下方法从其他B细胞中纯化出来：先用特异性抗原进行预孵育，然后与抗小鼠Ig偶联的红细胞进行花环形成，再在Ficoll-Isopaque上沉淀。该程序可去除大多数B细胞，而保留那些对孵育中所用抗原具有特异性的细胞。相对于B细胞含量，ASC富集了64至132倍。该方法具有高度特异性，因为针对两种不同抗原（火鸡γ球蛋白和绵羊红细胞）致敏的B细胞可以彼此分离。与直接获得ASC的纯化程序相比，这种间接纯化程序的优点在于获得ASC的方法简单，并且ASC能够在不添加其他细胞的情况下对抗原作出反应。

相似文献

1
Enrichment of antigen-specific B lymphocytes by the direct removal of B cells not bearing specificity for the antigen.通过直接去除对该抗原无特异性的B细胞来富集抗原特异性B淋巴细胞。
J Exp Med. 1977 Aug 1;146(2):445-56. doi: 10.1084/jem.146.2.445.
2
Separation of various B-cell subpopulations from mouse spleen. II. Depletion of antigen-specific B cells by rosetting with glutaraldehyde-fixed, antigen-coupled red blood cells.从小鼠脾脏中分离各种B细胞亚群。II. 用戊二醛固定、抗原偶联的红细胞进行玫瑰花结形成以耗尽抗原特异性B细胞。
Cell Immunol. 1979 Aug;46(1):170-7. doi: 10.1016/0008-8749(79)90254-5.
3
Separation of human B lymphocytes by combined rosetting with mouse and sheep red cells.通过与小鼠和绵羊红细胞联合玫瑰花结形成法分离人B淋巴细胞。
Arch Immunol Ther Exp (Warsz). 1980;28(1):45-51.
4
Ability of antigen-specific helper cells to effect a class-restricted increase in total Ig-secreting cells in spleens after immunization with the antigen.抗原特异性辅助细胞在经抗原免疫后使脾脏中总Ig分泌细胞产生类别受限增加的能力。
J Exp Med. 1979 Sep 19;150(3):517-30. doi: 10.1084/jem.150.3.517.
5
Relationship between Fc receptors, antigen-binding sites on T and B cells, and H-2 complex-associated determinants.Fc受体、T细胞和B细胞上的抗原结合位点与H-2复合体相关决定簇之间的关系。
J Exp Med. 1975 Mar 1;141(3):547-60. doi: 10.1084/jem.141.3.547.
6
A two-step centrifugation procedure for the purification of sheep erythrocyte antigen-binding cells.一种用于纯化绵羊红细胞抗原结合细胞的两步离心法。
J Immunol. 1978 Apr;120(4):1233-9.
7
The effect of second signals on the induction of B cell tolerance: failure of helper T cells to block tolerance induction.第二信号对B细胞耐受性诱导的影响：辅助性T细胞无法阻断耐受性诱导。
Eur J Immunol. 1981 Jul;11(7):550-6. doi: 10.1002/eji.1830110706.
8
Enumeration and isolation of human T and B lymphocytes by rosette formation with antibody-coated erythrocytes.通过与抗体包被红细胞形成玫瑰花结来计数和分离人T淋巴细胞和B淋巴细胞。
Clin Exp Immunol. 1975 Oct;22(1):62-71.
9
Inhibition of the immune response of mouse spleen cells to sheep erythrocytes in vitro by anti-immunoglobulin sera.抗免疫球蛋白血清对小鼠脾细胞体外抗绵羊红细胞免疫反应的抑制作用。
Eur J Immunol. 1971 Jun;1(3):157-62. doi: 10.1002/eji.1830010303.
10
Activation of human B lymphocytes. XVII. Synergy between nonspecific and specific signals in the antigen-specific responses of human B cells.人B淋巴细胞的激活。十七。人B细胞抗原特异性应答中非特异性信号与特异性信号之间的协同作用。
Cell Immunol. 1981 May 15;60(2):415-25. doi: 10.1016/0008-8749(81)90283-5.

引用本文的文献

1
Resistance to Nocardia brasiliensis infection in mice immunized with either Nocardia or BCG.用巴西诺卡菌或卡介苗免疫的小鼠对巴西诺卡菌感染的抵抗力。
Mycopathologia. 1980 Mar 17;70(2):117-22. doi: 10.1007/BF00443077.
2
In vitro tolerance induction of primed, IgD-negative murine spleen cells.致敏的、IgD 阴性小鼠脾细胞的体外耐受诱导
J Exp Med. 1981 Mar 1;153(3):653-64. doi: 10.1084/jem.153.3.653.
3
In vitro anamnestic immune responses and modulating factors.体外回忆性免疫反应及调节因子。
Mol Cell Biochem. 1979 Dec 14;28(1-3):107-34. doi: 10.1007/BF00223362.

本文引用的文献

1
Immunization of dissociated spleen cell cultures from normal mice.对来自正常小鼠的脾细胞解离培养物进行免疫接种。
J Exp Med. 1967 Sep 1;126(3):423-42. doi: 10.1084/jem.126.3.423.
2
Cell separation on antigen-coated columns. Elimination of high rate antibody-forming cells and immunological memory cells.抗原包被柱上的细胞分离。去除高速率抗体形成细胞和免疫记忆细胞。
J Exp Med. 1969 Jan 1;129(1):23-36. doi: 10.1084/jem.129.1.23.
3
Proliferative and differentiative manifestations of cellular immune potential.细胞免疫潜能的增殖和分化表现
Prog Allergy. 1967;10:1-36.
4
Cell fractionation and arrangement on fibers, beads, and surfaces.细胞分级分离以及在纤维、珠子和表面上的排列。
Proc Natl Acad Sci U S A. 1971 Sep;68(9):2153-7. doi: 10.1073/pnas.68.9.2153.
5
Fluorescence activated cell sorting.荧光激活细胞分选
Rev Sci Instrum. 1972 Mar;43(3):404-9. doi: 10.1063/1.1685647.
6
Protein A from Staphylococcus aureus. X. Complement-fixing activity of complexes between protein A and myeloma IgG or papain fragments of IgG.金黄色葡萄球菌的蛋白A。十、蛋白A与骨髓瘤IgG或IgG的木瓜蛋白酶片段之间复合物的补体固定活性。
J Immunol. 1970 Oct;105(4):944-8.
7
Separation of normal and immune lymphoid cells by antigen-coated coated columns. Antigen-binding characteristics of membrane antibodies as analyzed by hapten-protein antigens.通过抗原包被柱分离正常和免疫淋巴细胞。通过半抗原-蛋白质抗原分析膜抗体的抗原结合特性。
J Exp Med. 1970 Jul 1;132(1):110-26. doi: 10.1084/jem.132.1.110.
8
The mechanism of bone marrow-derived (B) lymphocyte activation. I. Early events in antigen-induced triggering in the presence of polymerized flagellin.骨髓来源的（B）淋巴细胞激活机制。I. 在聚合鞭毛蛋白存在下抗原诱导触发的早期事件。
Eur J Immunol. 1974 Jan;4(1):14-20. doi: 10.1002/eji.1830040105.
9
Events after the binding of antigen to lymphocytes: removal and regeneration of the antigen receptor.抗原与淋巴细胞结合后的事件：抗原受体的清除与再生
J Exp Med. 1974 May 1;139(5):1110-24. doi: 10.1084/jem.139.5.1110.
10
General method for isolation and recovery of B cells bearing specific receptors.携带特异性受体的B细胞的分离与回收通用方法。
J Exp Med. 1974 Mar 1;139(3):761-6. doi: 10.1084/jem.139.3.761.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型，支持多种主流文档格式。

立即体验