Hasselbacher P, Hahn J L
Atherosclerosis. 1980 Oct;37(2):239-45. doi: 10.1016/0021-9150(80)90009-x.
Microcrystalline cholesterol in either the anhydrous or monohydrate form was a potent activator of the alternative pathway of complement as measured by the electrophoretic conversion (crossed immunoelectrophoresis) of C3 and properdin factor B. Chelation with 0.01 M ethylene-diaminetetraacetate (EDTA) completely eliminated conversion, but 0.01 M ethyleneglycol tetraacetate (EGTA) had little or no effect. The magnitude of activation by cholesterol crystals was similar to that by zymosan, heat-aggregated IgG, or crystals of monosodium urate monohydrate. The microcrystalline forms of the acetate, linoleate, or oleate esters of cholesterol did not activate more complement than did saline controls. Cholestanol retained full C3 activating potency, but cholestane had none. Binding of IgG by cholesterol monohydrate is very small compared to that by sodium urate.
通过C3和备解素因子B的电泳转化（交叉免疫电泳）测定，无水或一水合物形式的微晶胆固醇是补体替代途径的有效激活剂。用0.01M乙二胺四乙酸（EDTA）螯合可完全消除转化，但0.01M乙二醇四乙酸（EGTA）几乎没有影响。胆固醇晶体的激活程度与酵母聚糖、热聚集IgG或一水合尿酸钠晶体的激活程度相似。胆固醇的醋酸酯、亚油酸酯或油酸酯的微晶形式激活补体的程度并不比生理盐水对照更高。胆甾烷醇保留了完全的C3激活能力，但胆甾烷则没有。与尿酸钠相比，一水合胆固醇对IgG的结合非常少。
