Doherty M, Whicher J T, Dieppe P A
Ann Rheum Dis. 1983 Jun;42(3):285-91. doi: 10.1136/ard.42.3.285.
Activation of serum C3 by monosodium urate monohydrate (MSU) crystals and other particles was determined by immunofixation following electrophoretic separation of C3 and its activation products. Densitometry allowed quantitation of results. MSU, hydroxyapatite, brushite, and calcium pyrophosphate dihydrate crystals split C3 under conditions which demonstrate activation via the alternative pathway (AP). Quantitatively similar results were obtained in immunoglobulin deficient serum. Activation was crystal specific and was reduced by heating, grinding, sonication, and aging of crystals. Other inflammatory particles (e.g., blackthorn) activated C3 via the AP: noninflammatory particles (e.g., diamond) caused insignificant activation. It is suggested that particle-induced activation of the alternative pathway of complement may be important in the initiation of crystal-induced synovitis.
通过对C3及其激活产物进行电泳分离后免疫固定，测定了单水尿酸钠（MSU）晶体和其他颗粒对血清C3的激活作用。光密度测定法可对结果进行定量分析。MSU、羟基磷灰石、透钙磷石和二水焦磷酸钙晶体在通过替代途径（AP）激活的条件下裂解C3。在免疫球蛋白缺陷血清中获得了定量相似的结果。激活具有晶体特异性，并且通过加热、研磨、超声处理和晶体老化而降低。其他炎性颗粒（如黑刺李）通过AP激活C3：非炎性颗粒（如钻石）引起的激活不明显。提示补体替代途径的颗粒诱导激活可能在晶体诱导性滑膜炎的起始中起重要作用。
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