3-羧基丙酰基-丙氨酰基-丙氨酰基-缬氨酸-4-硝基苯胺的合成与分析应用：一种人白细胞弹性蛋白酶的特异性底物
Synthesis and analytical use of 3-carboxypropionyl-alanyl-alanyl-valine-4-nitroanilide: a specific substrate for human leukocyte elastase.
作者信息
Wenzel H R, Engelbrecht S, Reich H, Mondry W, Tschesche H
出版信息
Hoppe Seylers Z Physiol Chem. 1980 Sep;361(9):1413-6. doi: 10.1515/bchm2.1980.361.2.1413.
PMID:6904347
Abstract
A simple synthesis is described for 3-carboxypropionyl-Ala-Ala-Val-4-nitroanilide, a convenient and very specific substrate for human leukocyte elastase (Km = 1.0mM, kcat = 8.7 s-1). The substrate does not undergo appreciable spontaneous hydrolysis. It is not cleaved by trypsin or chymotrypsin and only rather slowly by porcine pancreatic elastase (Km = 9.1mM, kcat = 1.4 s-1).
摘要
本文描述了3-羧基丙酰基-Ala-Ala-Val-4-硝基苯胺的一种简单合成方法，它是一种适用于人白细胞弹性蛋白酶的便捷且非常特异的底物（Km = 1.0mM，kcat = 8.7 s-1）。该底物不会发生明显的自发水解。它不会被胰蛋白酶或胰凝乳蛋白酶切割，而被猪胰弹性蛋白酶切割的速度相当缓慢（Km = 9.1mM，kcat = 1.4 s-1）。
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