Kramps J A, van Twisk C, van der Linden A C
Scand J Clin Lab Invest. 1983 Sep;43(5):427-32.
L-Pyroglutamyl-L-prolyl-L-valine-p-nitroanilide was found to be a highly specific substrate for human granulocyte elastase. At pH 8.3 and 37 degrees C, its Km = 0.55 mmol/l and the value for kcat was 6 sec-1, whereas with porcine pancreatic elastase these values were approximately 2 mmol/l and less than 0.001 sec-1, respectively. It is not cleaved by trypsin or chymotrypsin. With granulocyte elastase this new substrate is 50 times more sensitive compared to succinyltrialanyl-p-nitroanilide. L-Pyroglutamyl-L-prolyl-L-valine-p-nitroanilide can also be used for the assay of granulocyte elastase inhibitors.
L-焦谷氨酰-L-脯氨酰-L-缬氨酸对硝基苯胺被发现是人类粒细胞弹性蛋白酶的一种高度特异性底物。在pH 8.3和37摄氏度条件下，其米氏常数（Km）=0.55毫摩尔/升，催化常数（kcat）值为6秒-1，而对于猪胰弹性蛋白酶，这些值分别约为2毫摩尔/升和小于0.001秒-1。它不会被胰蛋白酶或糜蛋白酶切割。与琥珀酰丙氨酰对硝基苯胺相比，这种新底物对粒细胞弹性蛋白酶的敏感度要高50倍。L-焦谷氨酰-L-脯氨酰-L-缬氨酸对硝基苯胺也可用于粒细胞弹性蛋白酶抑制剂的测定。
