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对分离出的胶原纤维进行免疫电子显微镜研究。

Immunoelectronmicroscopical investigations on isolated collagen fibrils.

作者信息

Harnisch J P, Barrach H J, Sinha P K

出版信息

Albrecht Von Graefes Arch Klin Exp Ophthalmol. 1980;212(3-4):143-8. doi: 10.1007/BF00410510.

DOI:10.1007/BF00410510
PMID:6906129
Abstract

Isolated collagen fibrils treated with type-specific antibodies can be stained using a peroxidase-antibody complex. The staining pattern of the fibrils with this peroxidase-antibody complex is demonstrated and compared with the normal negative staining pattern of the fibrils. The possible binding sites of the anticollagen antibodies on the fibrils is also discussed.

摘要

用型特异性抗体处理过的分离胶原纤维可以使用过氧化物酶-抗体复合物进行染色。展示了这种过氧化物酶-抗体复合物对纤维的染色模式,并与纤维正常的负染色模式进行比较。还讨论了抗胶原抗体在纤维上可能的结合位点。

相似文献

1
Immunoelectronmicroscopical investigations on isolated collagen fibrils.对分离出的胶原纤维进行免疫电子显微镜研究。
Albrecht Von Graefes Arch Klin Exp Ophthalmol. 1980;212(3-4):143-8. doi: 10.1007/BF00410510.
2
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[Immunofluorescence microscopy studies with isolated type-specific antibodies against collagen type I, II and III in connective tissue diseases].[结缔组织病中针对I型、II型和III型胶原蛋白的分离型特异性抗体的免疫荧光显微镜研究]
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本文引用的文献

1
Procollagens.前胶原
Science. 1958 Jun 13;127(3311):1371-6. doi: 10.1126/science.127.3311.1371.
2
The staining pattern of collagen fibrils. I. An analysis of electron micrographs.胶原纤维的染色模式。I. 电子显微镜照片分析。
Connect Tissue Res. 1974;2(2):137-50. doi: 10.3109/03008207409152099.
3
Ultrastructural identification of type I and II collagen in the cornea of the mouse by means of enzyme labeled antibodies.利用酶标抗体对小鼠角膜中I型和II型胶原蛋白进行超微结构鉴定。
Albrecht Von Graefes Arch Klin Exp Ophthalmol. 1978 Nov 8;208(1-3):9-13. doi: 10.1007/BF00406977.
4
Antibodies to distinct types of collagens and procollagens and their application in immunohistology.
J Immunol Methods. 1977;18(1-2):165-82. doi: 10.1016/0022-1759(77)90168-5.