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利用酶标抗体对小鼠角膜中I型和II型胶原蛋白进行超微结构鉴定。

Ultrastructural identification of type I and II collagen in the cornea of the mouse by means of enzyme labeled antibodies.

作者信息

Harnisch J P, Buchen R, Sinha P K, Barrach H J

出版信息

Albrecht Von Graefes Arch Klin Exp Ophthalmol. 1978 Nov 8;208(1-3):9-13. doi: 10.1007/BF00406977.

DOI:10.1007/BF00406977
PMID:365012
Abstract

Following reaction with peroxidase-coupled IgG antibodies, collagen of both type I and type II can be demonstrated light and electron-microscopically in the cornea of the mouse by means of purified, type-specific antibodies against collagen. Both type I and type II collagen seem to be evenly distributed in the cornea, with the amount of type I exceeding that of type II.

摘要

在用过氧化物酶偶联的IgG抗体进行反应后,通过针对胶原蛋白的纯化的、类型特异性抗体,可在小鼠角膜中通过光学显微镜和电子显微镜观察到I型和II型胶原蛋白。I型和II型胶原蛋白似乎在角膜中均匀分布,I型胶原蛋白的量超过II型。

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Ultrastructural identification of type I and II collagen in the cornea of the mouse by means of enzyme labeled antibodies.利用酶标抗体对小鼠角膜中I型和II型胶原蛋白进行超微结构鉴定。
Albrecht Von Graefes Arch Klin Exp Ophthalmol. 1978 Nov 8;208(1-3):9-13. doi: 10.1007/BF00406977.
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本文引用的文献

1
Enzyme-labeled antibodies for the light and electron microscopic localization of tissue antigens.用于组织抗原光镜和电镜定位的酶标抗体。
J Cell Biol. 1967 May;33(2):307-18. doi: 10.1083/jcb.33.2.307.
2
Collagen heterogeneity in the avian eye: lens, vitreous body, cornea and sclera.鸟类眼睛中的胶原蛋白异质性:晶状体、玻璃体、角膜和巩膜。
Exp Eye Res. 1974 Apr;18(4):395-406. doi: 10.1016/0014-4835(74)90117-1.
3
[The proof of different types of collagen in the bovine eye (author's transl)].[牛眼不同类型胶原蛋白的证明(作者译)]
III型胶原蛋白在人类发育中牙齿牙髓实质中的分布。光镜和电镜免疫分型。
Histochemistry. 1982;74(3):319-28. doi: 10.1007/BF00493431.
4
Immunogold fine structural localization of extracellular matrix components in aged human cornea. I. Types I-IV collagen and laminin.老年人类角膜细胞外基质成分的免疫金精细结构定位。I. I型至IV型胶原蛋白和层粘连蛋白。
Graefes Arch Clin Exp Ophthalmol. 1991;229(2):157-63. doi: 10.1007/BF00170550.
Albrecht Von Graefes Arch Klin Exp Ophthalmol. 1975 Aug 4;196(1):71-7. doi: 10.1007/BF00410028.
4
Visualization of type I and II collagens in tissue sections by immunohistologic techniques.通过免疫组织学技术对组织切片中的I型和II型胶原蛋白进行可视化观察。
J Immunol. 1976 Jul;117(1):298-303.
5
Immunofluorescent localization of collagen types I, II, and III in the embryonic chick eye.I、II和III型胶原蛋白在鸡胚眼中的免疫荧光定位
Dev Biol. 1977 Aug;59(1):75-85. doi: 10.1016/0012-1606(77)90241-x.
6
Synthesis of two collagen types by embryonic chick corneal epithelium in vitro.胚胎鸡角膜上皮在体外合成两种胶原蛋白。
Proc Natl Acad Sci U S A. 1977 Jan;74(1):39-43. doi: 10.1073/pnas.74.1.39.