Evans R W, Setchell B P
J Reprod Fertil. 1978 Jul;53(2):357-62. doi: 10.1530/jrf.0.0530357.
Boar, bull and ram spermatozoa were studied after incubation in a medium containing phospholipids. Autoradiography and birefringent examination of spermatozoa after incubation with labelled phosphatidylcholine indicated that liposomes of this phospholipid associated with spermatozoa. The association was almost instantaneous. The large multilayer liposomes formed during the experimental procedure resulted in a few of the spermatozoa acquiring most of the phosphatidylcholine. Little phosphatidylcholine was observed unassociated with spermatozoa although measurements of the partition between spermatozoa and medium showed that only boar spermatozoa associated with almost all the phospholipid. No evidence was obtained that phosphatidylethanolamine or phosphatidylinositol associated with the spermatozoa.
对公猪、公牛和公羊的精子在含有磷脂的培养基中孵育后进行了研究。用标记的磷脂酰胆碱孵育精子后进行放射自显影和双折射检查，结果表明这种磷脂的脂质体与精子相关联。这种关联几乎是瞬间发生的。实验过程中形成的大型多层脂质体导致少数精子获取了大部分的磷脂酰胆碱。尽管对精子与培养基之间的分配进行测量显示只有公猪精子与几乎所有的磷脂相关联，但未观察到未与精子结合的磷脂酰胆碱。没有证据表明磷脂酰乙醇胺或磷脂酰肌醇与精子相关联。
