包膜型和裸露型痘苗病毒颗粒的吸附与穿透
Adsorption and penetration of enveloped and naked vaccinia virus particles.
作者信息
Payne L G, Norrby E
出版信息
J Virol. 1978 Jul;27(1):19-27. doi: 10.1128/JVI.27.1.19-27.1978.
Abstract
The adsorption and penetration of intracellular naked vaccinia virus (INV) and extracellular enveloped vaccinia virus (EEV) were examined. The adsorption kinetics of INV and EEV were similar, but INV adsorption was found to be more sensitive to the adsorption environment than EEV. The PFU-to-particle ratio for the two virus particles indicated that EEV was approximately two times as infectious as INV. Kinetic studies at 37 degree C showed that EEV penetrated cells more rapidly than INV. Penetration of EEV was unaffected by incubation in phsophate-buffered saline, but was somewhat reduced by incubation at 22 degree C. In contrast, INV penetration was effectively eliminated by incubation in phosphate-buffered saline or by incubation at 22 degree C. In addition, INV but not EEV pentration was sensitive to treatment with sodium fluoride and cytochalasin. B. These results are discussed with regard to the mechanism of INV and EEV penetration.
摘要
对细胞内裸痘苗病毒（INV）和细胞外包膜痘苗病毒（EEV）的吸附和穿透进行了检测。INV和EEV的吸附动力学相似，但发现INV吸附比EEV对吸附环境更敏感。两种病毒颗粒的蚀斑形成单位与颗粒比率表明，EEV的传染性约为INV的两倍。37℃下的动力学研究表明，EEV比INV更迅速地穿透细胞。EEV的穿透不受在磷酸盐缓冲盐溶液中孵育的影响，但在22℃下孵育会使其有所降低。相比之下，在磷酸盐缓冲盐溶液中孵育或在22℃下孵育可有效消除INV的穿透。此外，INV而非EEV的穿透对用氟化钠和细胞松弛素B处理敏感。针对INV和EEV的穿透机制对这些结果进行了讨论。
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