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转运核糖核酸中腺嘌呤和胞嘧啶残基与氯乙醛的反应。

The reaction of adenine and cytosine residues in tRNA with chloroacetaldehyde.

作者信息

Krzyzosiak W J, Biernat J, Ciesiołka J, Gulewicz K, Wiewiórowski M

出版信息

Nucleic Acids Res. 1981 Jun 25;9(12):2841-51. doi: 10.1093/nar/9.12.2841.

DOI:10.1093/nar/9.12.2841
PMID:6912442
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC326897/
Abstract

The reaction of Lupinus luteus tRNAPhe with 1 M chloroacetaldehyde in the pH range of 4 - 6 at 25 degrees C was studied. It was found that earlier difficulties lowering the utility of the reagent for structural studies of nucleic acids were caused by the formation of the stable reaction intermediates. In order to eliminate these difficulties the simple procedure of so-called maturation of the chloroacetaldehyde-modifies tRNA is proposed. It consists in further incubation of the short-term modified tRNA in water at 50 degrees C in the absence of the reagent. During the maturation step the stable intermediates are quantitatively converted into final ethenoderivatives. New HPLC conditions on Aminex A-6 were worked out which enable rapid separation of both ethenoadenosine and ethenocytidine from natural tRNA nucleosides.

摘要

研究了白羽扇豆苯丙氨酸转运核糖核酸（Lupinus luteus tRNAPhe）在25℃、pH值为4 - 6的条件下与1 M氯乙醛的反应。结果发现，早期在核酸结构研究中降低该试剂效用的困难是由稳定反应中间体的形成所致。为消除这些困难，提出了所谓氯乙醛修饰的转运核糖核酸“成熟”的简单程序。该程序包括在无试剂的情况下，将短期修饰的转运核糖核酸在50℃的水中进一步孵育。在成熟步骤中，稳定中间体被定量转化为最终的乙烯衍生物。制定了在Aminex A - 6上的新高效液相色谱（HPLC）条件，可实现乙烯腺苷和乙烯胞苷与天然转运核糖核酸核苷的快速分离。

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本文引用的文献

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Fluorescent adenosine and cytidine derivatives.荧光腺苷和胞苷衍生物
Biochem Biophys Res Commun. 1972 Jan 31;46(2):597-604. doi: 10.1016/s0006-291x(72)80181-5.
2
Chloroacetaldehyde-modified dinucleoside phosphates. Dynamic fluorescence quenching and quenching due to intramolecular complexation.氯乙醛修饰的二核苷磷酸。动态荧光猝灭及分子内络合导致的猝灭。
Biochemistry. 1974 Nov 19;13(24):4869-78. doi: 10.1021/bi00721a001.
3
Location of accessible bases in Escherichia coli formylmethionine transfer RNA as determined by chemical modification.通过化学修饰确定大肠杆菌甲酰甲硫氨酸转移RNA中可及碱基的位置
Biochemistry. 1976 Dec 28;15(26):5769-75. doi: 10.1021/bi00671a013.
4
New observations concerning the chloroacetaldehyde reaction with some tRNA constituents. Stable intermediates, kinetics and selectivity of the reaction.关于氯乙醛与某些tRNA成分反应的新观察结果。反应的稳定中间体、动力学和选择性。
Nucleic Acids Res. 1978 Mar;5(3):789-804. doi: 10.1093/nar/5.3.789.

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