大鼠线粒体大核糖体RNA基因及相邻tRNA基因的核苷酸序列。
The nucleotide sequence of the large ribosomal RNA gene and the adjacent tRNA genes from rat mitochondria.
作者信息
Saccone C, Cantatore P, Gadaleta G, Gallerani R, Lanave C, Pepe G, Kroon A M
出版信息
Nucleic Acids Res. 1981 Aug 25;9(16):4139-48. doi: 10.1093/nar/9.16.4139.
Abstract
We have sequenced the Eco R(1) fragment D from rat mitochondrial DNA. It contains one third of the tRNA (Val) gene (the remaining part has been sequenced from the 3' end of the Eco R(1) fragment A) the complete gene for the large mt 16S rRNA, the tRNA (Leu) gene and the 5' end of an unidentified reading frame. The mt gene for the large rRNA from rat has been aligned with the homologous genes from mouse and human using graphic computer programs. Hypervariable regions at the center of the molecule and highly conserved regions toward the 3' end have been detected. The mt gene for tRNA Leu is of the conventional type and its primary structure is highly conserved among mammals. The mt gene for tRNA(Val) shows characteristics similar to those of other mt tRNA genes but the degree of homology is lower. Comparative studies confirm that AGA and AGG are read as stop codons in mammalian mitochondria.
摘要
我们已对大鼠线粒体DNA的Eco R(1)片段D进行了测序。它包含三分之一的tRNA（Val）基因（其余部分已从Eco R(1)片段A的3'端进行了测序）、完整的大线粒体16S rRNA基因、tRNA（Leu）基因以及一个未鉴定阅读框的5'端。使用图形计算机程序将大鼠的大rRNA线粒体基因与小鼠和人类的同源基因进行了比对。检测到分子中心的高变区和靠近3'端的高度保守区。tRNA Leu的线粒体基因属于常规类型，其一级结构在哺乳动物中高度保守。tRNA(Val)的线粒体基因显示出与其他线粒体tRNA基因相似的特征，但同源程度较低。比较研究证实，AGA和AGG在哺乳动物线粒体中被解读为终止密码子。
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