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大肠杆菌16S和23S核糖体RNA的精细二级结构模型

Refined secondary structure models for the 16S and 23S ribosomal RNA of Escherichia coli.

作者信息

Maly P, Brimacombe R

出版信息

Nucleic Acids Res. 1983 Nov 11;11(21):7263-86. doi: 10.1093/nar/11.21.7263.

Abstract

The complete range of published sequences for ribosomal RNA (or rDNA), totalling well over 50,000 bases, has been used to derive refined models for the secondary structures of both 16S and 23S RNA from E. coli. Particular attention has been paid to resolving the differences between the various published secondary structures for these molecules. The structures are described in terms of 133 helical regions (45 for 16S RNA and 88 for 23S RNA). Of these, approximately 20 are still tentative or unconfirmed. A further 20 represent helical regions which definitely exist, but where the detailed base-pairing is still open to discussion. Over 90 of the helical regions are however now precisely established, at least to within one or two base pairs.

摘要

核糖体RNA(或rDNA)已发表序列的完整范围总计超过50000个碱基,已被用于推导大肠杆菌16S和23S RNA二级结构的优化模型。人们特别关注解决这些分子各种已发表二级结构之间的差异。这些结构是根据133个螺旋区域来描述的(16S RNA有45个,23S RNA有88个)。其中,约20个仍然是暂定的或未经证实的。另外20个代表肯定存在的螺旋区域,但具体的碱基配对仍有待讨论。然而,现在至少有90多个螺旋区域已精确确定,误差在一两个碱基对以内。

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