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人尿激肽释放酶和胰激肽释放酶的免疫学及酶学性质比较

Comparison of immunological and enzymatic properties of human urinary and pancreatic kallikrein.

作者信息

Fink E, Amouric M, Geiger R, Figarella C

出版信息

Hoppe Seylers Z Physiol Chem. 1982 Aug;363(8):819-23. doi: 10.1515/bchm2.1982.363.2.819.

Abstract

Human urinary and human pancreatic kallikreins were compared by radioimmunoassay, immunoelectrophoresis, by their enzymatic activity using the substrate Ac-Phe-Arg-OEt and by their biological activity employing the dog blood pressure assay. The only significant difference observed was a higher anodal mobility of the urinary kallikrein under the conditions of immunoelectrophoresis.

摘要

通过放射免疫测定法、免疫电泳、使用底物Ac-Phe-Arg-OEt测定其酶活性以及采用犬血压测定法评估其生物活性,对人尿激肽释放酶和人胰腺激肽释放酶进行了比较。观察到的唯一显著差异是在免疫电泳条件下,尿激肽释放酶具有更高的阳极迁移率。

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