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起始tRNA与真核起始因子eIF-2结合的调控。血红素控制的阻遏物对三元复合物形成动力学的影响。

Regulation of binding of initiator tRNA to eukaryotic initiation factor eIF-2. Effects of the haem-controlled repressor on the kinetics of ternary complex formation.

作者信息

Proud C G, Clemens M J, Pain V M

出版信息

FEBS Lett. 1982 Nov 8;148(2):214-20. doi: 10.1016/0014-5793(82)80810-7.

DOI:10.1016/0014-5793(82)80810-7
PMID:6924897
Abstract

Ternary complex formation was studied in reticulocyte lysate supernatants and using rat liver eukaryotic initiation factor-2 (eIF-2) preparations. Haem-deficiency reduced the rate of formation of ternary (Met-tRNAf . GTP . eIF-2) complexes by the eIF-2 in reticulocyte supernatants, the reduction being more marked when complex formation was assayed in the absence of GTP-regenerating capacity. Pretreatment with the haem-controlled repressor (HCR) reduced the rate of ternary complex formation by crude (liver) eIF-2. In contrast, complex formation by an almost homogeneous eIF-2 preparation was unaffected by HCR: sensitivity to HCR was however restored by a factor which catalyses exchange of guanine nucleotides bound to eIF-2.

摘要

在网织红细胞裂解物上清液中，并使用大鼠肝脏真核起始因子2（eIF-2）制剂研究了三元复合物的形成。血红素缺乏降低了网织红细胞上清液中eIF-2形成三元（Met-tRNAf·GTP·eIF-2）复合物的速率，当在缺乏GTP再生能力的情况下测定复合物形成时，这种降低更为明显。用血红素控制阻遏物（HCR）预处理降低了粗制（肝脏）eIF-2形成三元复合物的速率。相比之下，几乎纯一的eIF-2制剂形成复合物不受HCR影响：然而，与eIF-2结合的鸟嘌呤核苷酸催化交换的因子可恢复对HCR的敏感性。

相似文献

1
Regulation of binding of initiator tRNA to eukaryotic initiation factor eIF-2. Effects of the haem-controlled repressor on the kinetics of ternary complex formation.起始tRNA与真核起始因子eIF-2结合的调控。血红素控制的阻遏物对三元复合物形成动力学的影响。
FEBS Lett. 1982 Nov 8;148(2):214-20. doi: 10.1016/0014-5793(82)80810-7.
2
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引用本文的文献

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Mol Cell Biochem. 1998 Jun;183(1-2):69-78. doi: 10.1023/a:1006829615464.
2
Phosphorylation of the alpha subunit of initiation factor 2 correlates with the inhibition of translation following transient cerebral ischaemia in the rat.
Biochem J. 1994 Sep 1;302 ( Pt 2)(Pt 2):335-8. doi: 10.1042/bj3020335.
3
Effects of starvation, diabetes and acute insulin treatment on the regulation of polypeptide-chain initiation in rat skeletal muscle.饥饿、糖尿病及急性胰岛素治疗对大鼠骨骼肌中多肽链起始调控的影响。
Biochem J. 1984 Nov 1;223(3):687-96. doi: 10.1042/bj2230687.
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Biochem J. 1986 May 1;235(3):625-37. doi: 10.1042/bj2350625.
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Biochem J. 1988 Sep 15;254(3):773-9. doi: 10.1042/bj2540773.
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Biochem J. 1989 Aug 15;262(1):69-75. doi: 10.1042/bj2620069.
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