Bodwin J S, Clair T, Cho-Chung Y S
J Natl Cancer Inst. 1980 Feb;64(2):395-8. doi: 10.1093/jnci/64.2.395.
Estrogen-binding activity and the binding activity of adenosine 3',5'-cyclic monophosphate (cAMP) were assayed in the cytosol of biopsy specimens from 70 rat mammary tumors. The response of these tumors to host ovariectomy was followed. The hormone dependency of the tumors was closely related to the relative concentrations of estrogen receptor (ER) and cAMP-binding protein (CR) in the tumor cytosol. When ER and CR were expressed as the ER/CR ratio, 36 of 38 (95%) hormone-dependent tumors had ratios of 35 X 10(-3) or more, whereas 31 of 32 (97%) hormone-independent tumors had ratios of less than 35 X 10(-3). When ER alone was measured, hormone dependency could be correctly predicted in only 60% of the tumors because the ER range for individual tumors of both types greatly overlapped. Ratios of ER/CR were also elevated in normal estrogen target tissues as compared to those in normal estrogen nontarget tissues. Thus determination of the ER/CR ratio in tumor cytosols appeared to be a more reliable method of predicting hormone dependency than was determination of ER alone.
对70例大鼠乳腺肿瘤活检标本的胞质溶胶进行了雌激素结合活性和3',5'-环磷酸腺苷（cAMP）结合活性的测定。跟踪这些肿瘤对宿主卵巢切除术的反应。肿瘤的激素依赖性与肿瘤胞质溶胶中雌激素受体（ER）和cAMP结合蛋白（CR）的相对浓度密切相关。当将ER和CR表示为ER/CR比值时，38例激素依赖性肿瘤中的36例（95%）比值为35×10⁻³或更高，而32例激素非依赖性肿瘤中的31例（97%）比值小于35×10⁻³。仅测量ER时，仅60%的肿瘤能够正确预测激素依赖性，因为两种类型的单个肿瘤的ER范围有很大重叠。与正常非雌激素靶组织相比，正常雌激素靶组织中的ER/CR比值也升高。因此，与单独测定ER相比，测定肿瘤胞质溶胶中的ER/CR比值似乎是预测激素依赖性更可靠的方法。
