分化中的3T3-L1前脂肪细胞中胰岛素受体的合成与周转
Insulin receptor synthesis and turnover in differentiating 3T3-L1 preadipocytes.
作者信息
Reed B C, Lane M D
出版信息
Proc Natl Acad Sci U S A. 1980 Jan;77(1):285-9. doi: 10.1073/pnas.77.1.285.
Abstract
A density-shift method is described for analyzing insulin receptor synthesis and turnover in cultured cells labeled with "heavy" amino acids (2H, 13C, and 15N). Solubilized newly synthesized heavy and old "light" receptors are separated by isopycnic banding on CsCl gradients and then quantitated. Insulin receptor synthesis and turnover were studied by this technique in 3T3-L1 preadipocytes which undergo an increase in insulin binding capacity during differentiation. The results indicate that the increase in insulin binding capacity is a consequence of new receptor synthesis, that the insulin receptor has a relatively short half-life (6.7 hr), and that an increased rate of receptor synthesis contributes to the increase of insulin receptor level during differentiation.
摘要
描述了一种密度转移方法，用于分析用“重”氨基酸（2H、13C和15N）标记的培养细胞中胰岛素受体的合成和周转。通过在CsCl梯度上进行等密度沉降分离溶解的新合成的重受体和旧的“轻”受体，然后进行定量。利用该技术在3T3-L1前脂肪细胞中研究了胰岛素受体的合成和周转，这些细胞在分化过程中胰岛素结合能力增加。结果表明，胰岛素结合能力的增加是新受体合成的结果，胰岛素受体具有相对较短的半衰期（6.7小时），并且受体合成速率的增加有助于分化过程中胰岛素受体水平的增加。
相似文献
1
Insulin receptor synthesis and turnover in differentiating 3T3-L1 preadipocytes.分化中的3T3-L1前脂肪细胞中胰岛素受体的合成与周转
Proc Natl Acad Sci U S A. 1980 Jan;77(1):285-9. doi: 10.1073/pnas.77.1.285.
2
Insulin receptor synthesis and turnover in differentiating 3T3-L1 preadipocytes.分化中的3T3-L1前脂肪细胞中胰岛素受体的合成与更新
Prog Clin Biol Res. 1981;66 Pt A:523-42.
3
Kinetics of insulin receptor transit to and removal from the plasma membrane.胰岛素受体向质膜转运及从质膜移除的动力学。
J Biol Chem. 1983 Jan 10;258(1):283-90.
4
Control of insulin receptor level in 3T3 cells: effect of insulin-induced down-regulation and dexamethasone-induced up-regulation on rate of receptor inactivation.3T3细胞中胰岛素受体水平的调控：胰岛素诱导的下调和地塞米松诱导的上调对受体失活速率的影响。
Proc Natl Acad Sci U S A. 1982 May;79(9):2822-6. doi: 10.1073/pnas.79.9.2822.
5
Role of glycosylation and protein synthesis in insulin receptor metabolism by 3T3-L1 mouse adipocytes.糖基化和蛋白质合成在3T3-L1小鼠脂肪细胞胰岛素受体代谢中的作用
Proc Natl Acad Sci U S A. 1981 May;78(5):2908-12. doi: 10.1073/pnas.78.5.2908.
6
Alterations in insulin binding accompanying differentiation of 3T3-L1 preadipocytes.3T3-L1前脂肪细胞分化过程中胰岛素结合的变化。
Proc Natl Acad Sci U S A. 1977 Nov;74(11):4876-80. doi: 10.1073/pnas.74.11.4876.
7
Insulin-induced down-regulation of insulin receptors in 3T3-L1 adipocytes. Altered rate of receptor inactivation.胰岛素诱导3T3-L1脂肪细胞中胰岛素受体的下调。受体失活速率改变。
J Biol Chem. 1982 Apr 25;257(8):4285-91.
8
9
Post-translational glycosylation-induced activation of aglycoinsulin receptor accumulated during tunicamycin treatment.衣霉素处理期间积累的去糖基化胰岛素受体经翻译后糖基化诱导激活。
J Biol Chem. 1981 May 25;256(10):4704-7.
10
Up- and down-regulation of insulin receptors. Kinetic models.胰岛素受体的上调和下调。动力学模型。
J Biol Chem. 1984 Feb 25;259(4):2161-5.
引用本文的文献
1
Molecular determinants for the association of human hormone-sensitive lipase with lipid droplets.人激素敏感性脂肪酶与脂滴结合的分子决定因素。
Nat Commun. 2025 Apr 12;16(1):3497. doi: 10.1038/s41467-025-58887-z.
2
Silk Fibroin Nanoparticles as a Drug Delivery System of 3,3'-Diindolylmethane with Potential Antiobesogenic Activity.丝素蛋白纳米颗粒作为具有潜在抗肥胖活性的3,3'-二吲哚甲烷的药物递送系统
ACS Omega. 2024 Nov 20;9(48):47661-47671. doi: 10.1021/acsomega.4c07203. eCollection 2024 Dec 3.
3
Effects of mifepristone on adipocyte differentiation in mouse 3T3-L1 cells.米非司酮对小鼠 3T3-L1 脂肪细胞分化的影响。
Cell Mol Biol Lett. 2024 Mar 29;29(1):45. doi: 10.1186/s11658-024-00559-9.
4
Sialic acids cleavage induced by elastin-derived peptides impairs the interaction between insulin and its receptor in adipocytes 3T3-L1.弹性蛋白衍生肽诱导唾液酸裂解会损害脂肪细胞 3T3-L1 中胰岛素与其受体的相互作用。
J Physiol Biochem. 2024 May;80(2):363-379. doi: 10.1007/s13105-024-01010-5. Epub 2024 Feb 23.
5
Oncogenic KRAS Drives Lipofibrogenesis to Promote Angiogenesis and Colon Cancer Progression.致癌性 KRAS 驱动脂肪纤维生成以促进血管生成和结肠癌进展。
Cancer Discov. 2023 Dec 12;13(12):2652-2673. doi: 10.1158/2159-8290.CD-22-1467.
6
Immature ginger reduces triglyceride accumulation by downregulating Acyl CoA carboxylase and phosphoenolpyruvate carboxykinase-1 genes in 3T3-L1 adipocytes.嫩姜通过下调3T3-L1脂肪细胞中的酰基辅酶A羧化酶和磷酸烯醇式丙酮酸羧激酶-1基因来减少甘油三酯积累。
Food Nutr Res. 2023 Mar 2;67. doi: 10.29219/fnr.v67.9126. eCollection 2023.
7
A novel 3D culture model for human primary mammary adipocytes to study their metabolic crosstalk with breast cancer in lean and obese conditions.一种新型的人类原代乳腺脂肪细胞 3D 培养模型，用于研究在肥胖和消瘦状态下它们与乳腺癌的代谢串扰。
Sci Rep. 2023 Mar 22;13(1):4707. doi: 10.1038/s41598-023-31673-x.
8
Improved Protocol to Study Osteoblast and Adipocyte Differentiation Balance.研究成骨细胞与脂肪细胞分化平衡的改进方案
Biomedicines. 2022 Dec 22;11(1):31. doi: 10.3390/biomedicines11010031.
9
Rab8 attenuates Wnt signaling and is required for mesenchymal differentiation into adipocytes.Rab8减弱Wnt信号传导，并且是间充质细胞分化为脂肪细胞所必需的。
J Biol Chem. 2021 Jan-Jun;296:100488. doi: 10.1016/j.jbc.2021.100488. Epub 2021 Mar 1.
10
Mechanisms Mediating Anti-Inflammatory Effects of Delta-Tocotrienol and Tart Cherry Anthocyanins in 3T3-L1 Adipocytes.介导δ-生育酚和酸樱桃花色苷在 3T3-L1 脂肪细胞中抗炎作用的机制。
Nutrients. 2020 Oct 30;12(11):3356. doi: 10.3390/nu12113356.
本文引用的文献
1
Insulin-induced dissociation of its receptor into subunits: possible molecular concomitant of negative cooperativity.胰岛素诱导其受体解离成亚基：负协同性可能的分子伴随现象。
Biochem Biophys Res Commun. 1976 Dec 20;73(4):1068-74. doi: 10.1016/0006-291x(76)90232-1.
2
Properties of the insulin receptor isolated from liver and fat cell membranes.从肝细胞膜和脂肪细胞膜分离出的胰岛素受体的特性。
J Biol Chem. 1972 Apr 10;247(7):1980-91.
3
Isolation of the insulin receptor of liver and fat-cell membranes (detergent-solubilized-( 125 I)insulin-polyethylene glycol precipitation-sephadex).肝和脂肪细胞膜胰岛素受体的分离（去污剂增溶 - （125I）胰岛素 - 聚乙二醇沉淀 - 葡聚糖凝胶）
Proc Natl Acad Sci U S A. 1972 Feb;69(2):318-22. doi: 10.1073/pnas.69.2.318.
4
Spontaneous heritable changes leading to increased adipose conversion in 3T3 cells.导致3T3细胞脂肪转化增加的自发遗传性变化。
Cell. 1976 Jan;7(1):105-13. doi: 10.1016/0092-8674(76)90260-9.
5
Development of lipoprotein lipase in cultured 3T3-L1 cells.培养的3T3-L1细胞中脂蛋白脂肪酶的发育
Biochem Biophys Res Commun. 1977 Sep 9;78(1):288-93. doi: 10.1016/0006-291x(77)91252-9.
6
Insulin receptors in 3T3 fibroblasts. Relationship to growth phase, transformation and differentiation into new cell types.3T3成纤维细胞中的胰岛素受体。与生长阶段、转化以及分化为新细胞类型的关系。
Exp Cell Res. 1977 Jul;107(2):293-9. doi: 10.1016/0014-4827(77)90352-4.
7
Increasing activity of enzymes on pathway of triacylglycerol synthesis during adipose conversion of 3T3 cells.在3T3细胞脂肪转化过程中，甘油三酯合成途径中酶的活性增加。
J Biol Chem. 1977 Mar 25;252(6):2158-60.
8
Kinetics of biosynthesis of acetylcholine receptor and subsequent incorporation into plasma membrane of cultured chick skeletal muscle.培养的鸡骨骼肌中乙酰胆碱受体生物合成的动力学及随后整合到质膜中的过程。
Cell. 1977 Mar;10(3):365-73. doi: 10.1016/0092-8674(77)90023-x.
9
10
Differentiation of 3T3-L1 fibroblasts to adipocytes. Effect of insulin and indomethacin on the levels of insulin receptors.3T3-L1成纤维细胞向脂肪细胞的分化。胰岛素和吲哚美辛对胰岛素受体水平的影响。
J Biol Chem. 1978 Jul 10;253(13):4693-6.
Zotero 插件