鱿鱼神经丝的多肽组成。

Roslansky P F, Cornell-Bell A, Rice R V, Adelman W J

Proc Natl Acad Sci U S A. 1980 Jan;77(1):404-8. doi: 10.1073/pnas.77.1.404.

Neurofilaments, 10 nm in diameter, from the axoplasm of the squid Loligo pealei have been isolated by a combination of sonication and Millipore filtration. The presence of neurofilaments during the isolation procedure was confirmed by negative staining and transmission electron microscopy. By use of this technique, which results in minimal or no chemical alteration of the native neurofilament proteins, it was shown that actin (43,000 daltons) and tubulin (56,000 daltons) are physically separable from intact neurofilaments. The neurofilament-rich retentate contained two major proteins of 200,000 and 63,000 daltons and larger polypeptides.

通过超声处理和微孔过滤相结合的方法，从枪乌贼的轴浆中分离出了直径为10纳米的神经丝。在分离过程中，通过负染色和透射电子显微镜证实了神经丝的存在。使用这种技术，原生神经丝蛋白的化学改变极小或没有改变，结果表明肌动蛋白（43000道尔顿）和微管蛋白（56000道尔顿）在物理上可与完整的神经丝分离。富含神经丝的截留物包含两种主要蛋白质，分别为200000道尔顿和63000道尔顿，以及更大的多肽。