鱿鱼脑中间丝（神经丝）的体外重组装：通过组装-拆卸进行纯化。

In vitro reassembly of squid brain intermediate filaments (neurofilaments): purification by assembly-disassembly.

作者信息

Zackroff R V, Goldman R D

出版信息

Science. 1980 Jun 6;208(4448):1152-5. doi: 10.1126/science.7189605.

Abstract

Intermediate filaments from squid brain tissue were reassembled in vitro and purified by two cycles of assembly and disassembly. Purified squid brain filaments contained one major polypeptide (60,000 daltons), which constituted about 70 percent of the total protein, and three minor polypeptides (74,000, 100,000, and 220,000 daltons). Squid brain intermediate filaments were reconstituted from rod-shaped protofilamentous subunits. In addition to the intermediate filaments, dense bodies which may function in intermediate filament nucleation or organization were retained through two purification cycles.

摘要

来自鱿鱼脑组织的中间丝在体外进行了重装配，并通过两轮装配和拆卸进行了纯化。纯化后的鱿鱼脑中间丝包含一种主要多肽（60,000道尔顿），约占总蛋白的70%，以及三种次要多肽（74,000、100,000和220,000道尔顿）。鱿鱼脑中间丝由杆状原纤维亚基重构而成。除了中间丝外，在两轮纯化过程中都保留了可能在中间丝成核或组织中起作用的致密体。