Finbow M, Yancey S B, Johnson R, Revel J P
Proc Natl Acad Sci U S A. 1980 Feb;77(2):970-4. doi: 10.1073/pnas.77.2.970.
Several polypeptides have been described in the past as components of gap junction fractions. Of these, a peptide of Mr 26,000 is found in gap junctions isolated from livers of different species under conditions that minimize proteolysis. Tryptic digestion of purified "intact" junctions causes the rapid disappearance of this peptide with a concomitant appearance of a band of Mr 10,000, which has previously been found to be characteristic of junctional fractions isolated with the aid of proteolytic treatment. Both the peptides of Mr 26,000 and 10,000 are missing from gap junction preparations after partial hepatectomy, when gap junctions are absent from the surface of the hepatocytes. They have reappeared in fractions from the livers of animals killed 3 days postoperatively, when gap junctions are again present in vivo.
过去已有几种多肽被描述为间隙连接组分。其中，在不同物种肝脏中分离出的间隙连接中，在使蛋白水解降至最低的条件下发现了一种分子量为26,000的肽。对纯化的“完整”连接进行胰蛋白酶消化会导致该肽迅速消失，同时出现一条分子量为10,000的条带，此前已发现该条带是借助蛋白水解处理分离出的连接组分的特征。在部分肝切除术后，当肝细胞表面不存在间隙连接时，间隙连接制剂中分子量为26,000和10,000的肽均缺失。当术后3天处死的动物肝脏中的组分再次出现间隙连接时，它们又重新出现了。
