Revel J P, Yancey S B, Meyer D J, Nicholson B
In Vitro. 1980 Dec;16(12):1010-7. doi: 10.1007/BF02619251.
We have compared intercellular communication in normal and regenerating rat liver. Gap junctions are greatly reduced in size and numbers 29 to 35 hr after hepatectomy, but we still find some 90% of hepatocytes coupled by electrophysiological criteria. The spread of dyes such as carboxyfluorescein however is very limited in the regenerating organs as compared to the situation in the controls. We show how the apparent discrepancies between morphological and physiological data can be reconciled. We also present a summary of preliminary findings on the biosynthesis of gap junction protein and some of the conclusions one can draw from the sequence of 58 amino acids at the amino terminal of the protein.
我们比较了正常大鼠肝脏和再生大鼠肝脏中的细胞间通讯。肝切除术后29至35小时，缝隙连接的大小和数量大幅减少，但根据电生理标准，我们仍发现约90%的肝细胞相互耦联。然而，与对照组相比，诸如羧基荧光素等染料在再生器官中的扩散非常有限。我们展示了如何协调形态学和生理学数据之间明显的差异。我们还总结了缝隙连接蛋白生物合成的初步研究结果，以及从该蛋白氨基末端的58个氨基酸序列中可以得出的一些结论。
