Bondue H, Machin D, Stryckmans P A
Scand J Haematol. 1980 Jan;24(1):51-6. doi: 10.1111/j.1600-0609.1980.tb01317.x.
The marrow myeloid precursor cells of a haematologically normal patient were labelled by intravenous injection of tritiated thymidine. Young labelled segmented neutrophils were then released from the marrow into the blood by an injection of cortisol. These PMN had a significantly lower LAP activity than the older blood neutrophils. It is shown that within the morphologic boundaries of the segmented PMN, the cells are still in different stages of cytoplasmic maturation. In addition, labelled and unlabelled neutrophils showed a linear and parallel increase of LAP activity during the 24 h following cortisol injection. This observation suggests that the neutrophil prematurely released in the blood can mature into normal LAP=PMN and more generally that LAP activity of a blood neutrophil increases with time.
通过静脉注射氚标记的胸腺嘧啶核苷，对血液学正常患者的骨髓髓系前体细胞进行标记。然后通过注射皮质醇，使标记的年轻分叶中性粒细胞从骨髓释放到血液中。这些中性粒细胞碱性磷酸酶（PMN）的活性显著低于较老的血液中性粒细胞。结果表明，在分叶PMN的形态学界限内，细胞仍处于细胞质成熟的不同阶段。此外，在注射皮质醇后的24小时内，标记和未标记的中性粒细胞的中性粒细胞碱性磷酸酶活性呈线性且平行增加。这一观察结果表明，过早释放到血液中的中性粒细胞可成熟为正常的中性粒细胞碱性磷酸酶阳性的PMN，更普遍地说，血液中性粒细胞的中性粒细胞碱性磷酸酶活性随时间增加。
