Melamede R J, Wallace S S
Mol Gen Genet. 1980 Feb;177(3):501-9. doi: 10.1007/BF00271490.
The rate at which 3H thymidine is incorporated into DNA is increased in T4w-infected cells compared to wild-type when measured late in infection under conditions of low thymidine concentration. This increased DNA synthesis is sensitive to hydroxyurea but not to mitomycin C, and can be prevented by the addition of chloramphenicol early in infection. Also, DNA replicative intermediates isolated from T4w-infected cells late in infection sediment significantly faster than those isolated from wild-type-infected cells. In contrast, DNA replicative intermediates isolated from T4x- or T4y-infected cells sediment more slowly than those produced by wild-type T4. Cells coinfected with wild-type T4+ and T4x, y or w; or T4w and T4x or y, produce wild-type DNA replicative intermediates. Cells coinfected with T4x and T4y produce more slowly sedimenting DNA replicative intermedites. Cells coinfected with T4w and wild-type T4 show wild-type rates of DNA synthesis while cells coinfected with T4w and T4x or T4y show increased rates of DNA synthesis over that observed with wild-type alone.
在低胸苷浓度条件下于感染后期进行测量时，与野生型相比，T4w感染的细胞中3H胸苷掺入DNA的速率有所增加。这种DNA合成增加对羟基脲敏感，但对丝裂霉素C不敏感，并且在感染早期添加氯霉素可阻止其发生。此外，在感染后期从T4w感染的细胞中分离出的DNA复制中间体的沉降速度明显快于从野生型感染的细胞中分离出的中间体。相比之下，从T4x或T4y感染的细胞中分离出的DNA复制中间体沉降速度比野生型T4产生的中间体更慢。用野生型T4 +与T4x、y或w共感染的细胞；或T4w与T4x或y共感染的细胞，产生野生型DNA复制中间体。用T4x和T4y共感染的细胞产生沉降速度更慢的DNA复制中间体。用T4w和野生型T4共感染的细胞显示出野生型的DNA合成速率，而用T4w和T4x或T4y共感染的细胞显示出比单独野生型观察到的DNA合成速率更高。
