Gracheva N D, Danilova O A
Ontogenez. 1978;9(4):345-54.
To study the time of cell origin in the supraoptic nucleus, double labelling with 3H- and 14C-thymidine was applied to the matrix cells of embryonic ependyma with intervals of 12, 24 and 48 hrs from the 9th day of gestation. The localization of the labelled cells was studied in the brain of the offspring using autoradiography. The labelling allowed to determine the time of the last division of the matrix cells, prior to their differentiation into neurosecretory cells. The neurosecretory cells forming the supraoptic nucleus were shown to arise mainly on the 11--13th day of embryogenesis. The cells originated on the 11th day were located only in the dorso-caudal parts of the nucleus. The most neurosecretory cells arise in the second half of the 12th day and are distributed all over the nucleus with the dorso-ventral (caudo-rostral) gradient. From the 13th day on the migration of the neurosecretory cells in the supraoptic nucleus sharply reduces but still persits until the 17th day. The glioblasts begin to migrate in the supraoptic nucleus from the 14th day on.
为研究视上核中细胞起源的时间，在妊娠第9天起，以12小时、24小时和48小时的间隔，对胚胎室管膜的基质细胞进行³H-胸腺嘧啶核苷和¹⁴C-胸腺嘧啶核苷双标记。使用放射自显影术在子代的脑中研究标记细胞的定位。该标记能够确定基质细胞在分化为神经分泌细胞之前最后一次分裂的时间。形成视上核的神经分泌细胞主要在胚胎发育的第11 - 13天产生。在第11天起源的细胞仅位于核的背尾部分。大多数神经分泌细胞在第12天的后半期产生，并以背腹（尾头）梯度分布于整个核内。从第13天起，视上核中神经分泌细胞的迁移急剧减少，但一直持续到第17天。从第14天起，成胶质细胞开始向视上核迁移。
