长臂猿急性淋巴细胞白血病中的末端转移酶
Terminal transferase in acute lymphoblastic leukemia in gibbons.
作者信息
Sarin P S, Virmani M, Friedman B
出版信息
Biochim Biophys Acta. 1980 Jun 27;608(1):62-71. doi: 10.1016/0005-2787(80)90134-3.
Terminal deoxynucleotidyl transferase, an enzyme which catalyzes the polymerization of deoxyribonucleoside triphosphates on a 3'-OH end of an initiator molecule in the absence of a template, has been suggested as a biological marker for human acute lymphoblastic leukemia. Examination of a cell line, 6G1, recently established from the peripheral blood of a gibbon ape with acute lymphoblastic leukemia showed the presence of terminal deoxynucleotidyl transferase. This enzyme after purification by successive column chromatography on DEAE-cellulose, phosphocellulose and hydroxyapatite, was found to have biochemical properties similar to those reported for terminal transferase from calf thymus and human leukemic cells. These studies suggest that terminal transferase can be used as a useful biological marker for acute lymphoblastic leukemia in both humans and subhuman primates.
末端脱氧核苷酸转移酶是一种在没有模板的情况下催化脱氧核糖核苷三磷酸在引发分子的3'-OH末端聚合的酶，它被认为是人类急性淋巴细胞白血病的一种生物学标志物。对最近从一只患有急性淋巴细胞白血病的长臂猿外周血中建立的细胞系6G1进行检测，发现了末端脱氧核苷酸转移酶的存在。该酶经DEAE-纤维素、磷酸纤维素和羟基磷灰石柱层析连续纯化后，其生化特性与从小牛胸腺和人类白血病细胞中提取的末端转移酶所报道的特性相似。这些研究表明，末端转移酶可作为人类和亚人类灵长类动物急性淋巴细胞白血病的一种有用的生物学标志物。
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