小牛胸腺和人白血病细胞的末端脱氧核苷酸转移酶在固相免疫吸附剂上的亲和层析。
Affinity chromatography of terminal deoxynucleotidyl tranferase from calf thymus and human leukemic cells on a solid-phase immunoadsorbent.
作者信息
Srivastava B I, Chan J Y, Siddiqui F A
出版信息
J Biochem Biophys Methods. 1980 Jan-Feb;2(1):1-9. doi: 10.1016/0165-022x(80)90068-8.
A solid-phase immunoadsorbent specific for terminal deoxynucleotidyl transferase has been prepared. The enzyme from calf thymus and acute lymphoblastic leukemia cells binds to columns of this material. Bound enzyme can be eluted in an active form. Selective and rapid purification of terminal deoxynucleotidyl transferase from crude extracts of cells containing this enzyme can be achieved by this method since the immunoadsorbent has no affinity for other cellular DNA polymerases.
已制备出一种对末端脱氧核苷酸转移酶具有特异性的固相免疫吸附剂。来自小牛胸腺和急性淋巴细胞白血病细胞的该酶能与这种材料的柱体结合。结合的酶可以以活性形式洗脱下来。由于这种免疫吸附剂对其他细胞DNA聚合酶没有亲和力，因此通过这种方法可以从含有该酶的细胞粗提物中选择性快速地纯化末端脱氧核苷酸转移酶。
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