Akusjärvi G, Mathews M B, Andersson P, Vennström B, Pettersson U
Proc Natl Acad Sci U S A. 1980 May;77(5):2424-8. doi: 10.1073/pnas.77.5.2424.
A DNA sequence, 552 base pairs in length, encoding the two "virus-associated" (VA) RNAs of adenovirus type 2 is presented. Comparison of the oligonucleotide maps of VA RNAI and VA RNII with the established sequence permits identification of the genes for these RNAs. VA RNAI is 157-160 nucleotides long and VA RNAII 158-163 nucleotides long, depending on the exact length of their heterogeneous 3' end. The genes are separated by a spacer of about 98 nucleotides. The RNAs exhibit scattered regions of primary sequence homology and can adopt secondary structures which resemble each other closely in their configuration and stability. VA RNAII is also capable of assuming a different configuration that is energetically more favorable. The data suggest that the two RNA genes may have arisen by duplication of an ancestral gene and that the folding of the RNA chain may be of importance for the function of VA RNAs. Hypothetical RNA polymerase III recognition sequences and the coding potential of the region are discussed.
本文给出了一段长度为552个碱基对的DNA序列，该序列编码腺病毒2型的两种“病毒相关”（VA）RNA。通过将VA RNAI和VA RNII的寡核苷酸图谱与已确定的序列进行比较，可以确定这些RNA的基因。VA RNAI长度为157 - 160个核苷酸，VA RNII长度为158 - 163个核苷酸，具体长度取决于其异质3'末端的确切长度。这两个基因由大约98个核苷酸的间隔区隔开。这两种RNA表现出分散的一级序列同源区域，并且能够形成二级结构，其构型和稳定性彼此非常相似。VA RNAII也能够呈现出一种能量上更有利的不同构型。数据表明，这两个RNA基因可能是由一个祖先基因的复制产生的，并且RNA链的折叠可能对VA RNA的功能很重要。文中还讨论了假设的RNA聚合酶III识别序列以及该区域的编码潜力。
