胞质溶胶中一种增强激素刺激的腺苷酸环化酶活性的因子的特性。
Properties of a factor in cytosol that enhances hormones-stimulated adenylate cyclase activity.
作者信息
Crawford A, MacNeil S, Amirrasooli H, Tomlinson S
出版信息
Biochem J. 1980 May 15;188(2):401-7. doi: 10.1042/bj1880401.
Abstract
- The addition of 50 000g cytosol preparations of isolated human platelets, cultured rat osteogenic sarcoma or cultured bone cells to particulate preparations of adenylate cyclase, from the same or unrelated tissues, caused marked enhancement of the hormone-stimulated enzyme activities. 2. The degree of enhancement obtained by addition of the cytosol preparations was similar to that observed on addition of GTP. 3. The enhancing activity of the three cytosol types was found to be sensitive to digestion by trypsin and alkaline phosphatase, partially heat-labile and partially inactivated by exposure to charcoal. 4. Gel filtration studies indicated an apparent molecular weight of 20 000--30 000. Further, the 20000-30000-mol.wt. fractions obtained by gel filtration could enhance the adenylate cyclase activity of particulate preparations derived from unrelated cell types. 5. The results suggest a common or similar adenylate-cyclase-enhancing factor or factors, protein in nature, present in the three cytosol types.
摘要
- 将分离的人血小板、培养的大鼠骨肉瘤或培养的骨细胞的50000g胞质溶胶制剂添加到来自相同或不相关组织的腺苷酸环化酶颗粒制剂中，会导致激素刺激的酶活性显著增强。2. 添加胞质溶胶制剂所获得的增强程度与添加GTP时观察到的相似。3. 发现这三种胞质溶胶类型的增强活性对胰蛋白酶和碱性磷酸酶的消化敏感，部分对热不稳定，并且部分因接触活性炭而失活。4. 凝胶过滤研究表明表观分子量为20000 - 30000。此外，通过凝胶过滤获得的20000 - 30000分子量级分可以增强源自不相关细胞类型的颗粒制剂的腺苷酸环化酶活性。5. 结果表明在这三种胞质溶胶类型中存在一种共同或相似的、本质上为蛋白质的腺苷酸环化酶增强因子。
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