人关节软骨细胞的腺苷酸环化酶。对前列腺素和其他激素的反应性。
Adenylate cyclase of human articular chondrocytes. Responsiveness to prostaglandins and other hormones.
作者信息
Houston J P, McGuire M K, Meats J E, Ebsworth N M, Russell R G, Crawford A, Mac Neil S
出版信息
Biochem J. 1982 Oct 15;208(1):35-42. doi: 10.1042/bj2080035.
Adenylate cyclase [ATP pyrophosphate lyase (cyclizing), EC 4.6.1.1] was shown to be present in cultured human articular chondrocytes. Optimal conditions of incubation time, protein and substrate concentrations and pH were determined in whole cell lysates. Maximal activity occurred at pH 8.5 with no decrease in activity up to pH 10.0. Adenylate cyclase activity of particulate membrane preparations was enhanced by the addition of crude cytosol preparations. The prostaglandins E1, E2, F1 alpha, F2 alpha, D2, B1, B2, A1 and A2, as well as adrenaline and isoprenaline, stimulated adenylate cyclase derived from either adult or foetal chondrocytes. No significant stimulation was observed in the presence of human calcitonin or glucagon. Bovine parathyroid hormone always significantly stimulated the adenylate cyclase derived from foetal chondrocytes, but not from adult chondrocytes. Preincubation of the chondrocytes in culture with indomethacin and with or without supernatant medium from cultured mononuclear cells increased the responsiveness of the adenylate cyclase to prostaglandin E1.
腺苷酸环化酶[ATP焦磷酸裂解酶（环化），EC 4.6.1.1]已被证实在培养的人关节软骨细胞中存在。在全细胞裂解物中确定了孵育时间、蛋白质和底物浓度以及pH的最佳条件。最大活性出现在pH 8.5时，直至pH 10.0活性都没有下降。通过添加粗制胞质溶胶制剂可增强微粒体膜制剂的腺苷酸环化酶活性。前列腺素E1、E2、F1α、F2α、D2、B1、B2、A1和A2，以及肾上腺素和异丙肾上腺素，均可刺激来自成人或胎儿软骨细胞的腺苷酸环化酶。在人降钙素或胰高血糖素存在的情况下未观察到明显刺激作用。牛甲状旁腺激素总是能显著刺激来自胎儿软骨细胞的腺苷酸环化酶，但对成人软骨细胞来源的腺苷酸环化酶则无此作用。在培养中用吲哚美辛以及有或没有来自培养的单核细胞的上清培养基对软骨细胞进行预孵育，可增加腺苷酸环化酶对前列腺素E1的反应性。
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