Hirano H, Urist M R
J Natl Cancer Inst. 1980 Aug;65(2):299-309.
A cell line designated OSHU-1 was produced by cell culture of a grade II chondrosarcoma excised as a whole from the public bone of a 59-year-old man. The OSHU-1 chondrosarcoma cell line was maintained in vitro for over 10 months and was invariably tumorigenic when injected in inbred BALB/c athymic nude mice. The karyotype was grossly abnormal and suggestive of a malignant growth. Scanning and transmission electron microscopy showed features commonly associated with malignant transformation. Transplants of the isolated tumor cells reproduced many of the microscopic, histochemical, and biosynthetic features of the original chondrosarcoma. Hydroxyproline and uronic acid rapidly accumulated between 2 and 3 weeks after transplantation. 35S uptake increased about 5,5-fold within 7 days after transplantation. After 4-6 weeks in athymic mice, the rate of growth of the chondrosarcoma reached a plateau. The interior of the tumor calcified, while on the surface the tumor induced the formation of new bone and bone marrow, apparently of host origin.
一种名为OSHU-1的细胞系是通过对一名59岁男性耻骨上完整切除的II级软骨肉瘤进行细胞培养产生的。OSHU-1软骨肉瘤细胞系在体外维持了10多个月，当注射到近交系BALB/c无胸腺裸鼠体内时始终具有致瘤性。其核型明显异常，提示恶性生长。扫描和透射电子显微镜显示出与恶性转化相关的常见特征。分离出的肿瘤细胞移植再现了原始软骨肉瘤的许多微观、组织化学和生物合成特征。移植后2至3周内，羟脯氨酸和糖醛酸迅速积累。移植后7天内，35S摄取量增加了约5.5倍。在无胸腺小鼠中饲养4至6周后，软骨肉瘤的生长速度达到平稳期。肿瘤内部发生钙化，而在表面肿瘤诱导形成新骨和骨髓，显然源自宿主。
