Guigues M, Leng M
Nucleic Acids Res. 1979 Feb;6(2):733-44. doi: 10.1093/nar/6.2.733.
N-(guanosin-8-yl) acetylaminofluorene (Guo-AAF) was prepared by the reaction of N-acetoxy-N-2-acetylaminofluorene (AAAF) and guanosine. Antibodies to Guo-AAF were elicited in rabbits by immunization with bovine serum albumin-Guo-AAF conjugate. The antibodies were purified by affinity chromatography on a Sepharose-Guo-AAF column. The reactivity of these antibodies towards several ligands was studied by radioimmunoassay. The antibodies have the same affinity for double stranded DNA-AAF and single stranded DNA-AAF. Thus the geometry of the regions of DNA substituted by AAF residues is the same in native and denatured DNA. The affinity of the antibodies is smaller for DNA-AAF than for Guo-AAF. This can be due in part to the stacking of AAF residues with the adjacent bases as shown by the study of the interactions between the antibodies and AAF-oligonucleotides. The circular dichroism spectra of AAF-oligonucleotides bound to the antibodies are reported.
N-（鸟苷-8-基）乙酰氨基芴（Guo-AAF）通过N-乙酰氧基-N-2-乙酰氨基芴（AAAF）与鸟苷反应制备。通过用牛血清白蛋白-Guo-AAF缀合物免疫在兔中引发针对Guo-AAF的抗体。抗体通过在琼脂糖凝胶-Guo-AAF柱上的亲和色谱法纯化。通过放射免疫测定研究了这些抗体对几种配体的反应性。这些抗体对双链DNA-AAF和单链DNA-AAF具有相同的亲和力。因此，在天然和变性DNA中，被AAF残基取代的DNA区域的几何形状是相同的。抗体对DNA-AAF的亲和力比对Guo-AAF的亲和力小。这部分可能是由于AAF残基与相邻碱基的堆积，如对抗体与AAF-寡核苷酸之间相互作用的研究所表明的。报道了与抗体结合的AAF-寡核苷酸的圆二色光谱。
