基于结构同源性的触珠蛋白重链模型。
Model for haptoglobin heavy chain based upon structural homology.
作者信息
Greer J
出版信息
Proc Natl Acad Sci U S A. 1980 Jun;77(6):3393-7. doi: 10.1073/pnas.77.6.3393.
Abstract
A model has been constructed for haptoglobin heavy chain by using the known sequence homology to the mammalian serine proteases. The three-dimensional structures for three serine proteases, chymotrypsin, trypsin, and elastase, were compared and the structural features that are conserved in all three were extracted. The haptoglobin heavy chain sequence was aligned to the sequences of the three serine proteases by maximizing sequence homology in the regions of conserved structure. The resulting alignment shows that haptoglobin heavy chain must be very closely homologous to these proteases in structure as well as in sequence. Coordinates were derived for the heavy chain by using the homologous structures. The problems associated with these coordinates are outlined and methods for solving them are indicated. The features of the haptoglobin heavy chain structure are described. Implications of the structure for the very strong interaction between this subunit and hemoglobin are discussed.
摘要
通过利用与哺乳动物丝氨酸蛋白酶已知的序列同源性，构建了触珠蛋白重链模型。比较了三种丝氨酸蛋白酶（胰凝乳蛋白酶、胰蛋白酶和弹性蛋白酶）的三维结构，并提取了在这三种酶中都保守的结构特征。通过在保守结构区域最大化序列同源性，将触珠蛋白重链序列与这三种丝氨酸蛋白酶的序列进行比对。结果比对表明，触珠蛋白重链在结构和序列上都必须与这些蛋白酶非常紧密地同源。通过使用同源结构得出了重链的坐标。概述了与这些坐标相关的问题，并指出了解决这些问题的方法。描述了触珠蛋白重链结构的特征。讨论了该结构对于该亚基与血红蛋白之间非常强的相互作用的意义。
相似文献
1
Model for haptoglobin heavy chain based upon structural homology.基于结构同源性的触珠蛋白重链模型。
Proc Natl Acad Sci U S A. 1980 Jun;77(6):3393-7. doi: 10.1073/pnas.77.6.3393.
2
Covalent structure of human haptoglobin: a serine protease homolog.人触珠蛋白的共价结构：一种丝氨酸蛋白酶同源物。
Proc Natl Acad Sci U S A. 1980 Jun;77(6):3388-92. doi: 10.1073/pnas.77.6.3388.
3
Evidence of homology between the beta-chain of human haptoglobin and the chymotrypsin family of serine proteases.人触珠蛋白β链与丝氨酸蛋白酶胰凝乳蛋白酶家族之间的同源性证据。
Biochem Genet. 1974 Apr;11(4):279-93. doi: 10.1007/BF00485995.
4
Hemoglobin-binding site on haptoglobin probed by selective proteolysis.通过选择性蛋白酶解探究触珠蛋白上的血红蛋白结合位点。
J Biol Chem. 1983 Jan 25;258(2):1227-34.
5
Hemoglobin binding site and its relationship to the serine protease-like active site of haptoglobin.血红蛋白结合位点及其与触珠蛋白丝氨酸蛋白酶样活性位点的关系。
J Biol Chem. 1982 Sep 10;257(17):10063-8.
6
Tertiary structural differences between microbial serine proteases and pancreatic serine enzymes.微生物丝氨酸蛋白酶与胰腺丝氨酸酶之间的三级结构差异。
Nature. 1975 Oct 30;257(5529):758-63. doi: 10.1038/257758a0.
7
Structural similarities among concanavalin A, haptoglobin, and trypsin.伴刀豆球蛋白A、触珠蛋白和胰蛋白酶之间的结构相似性。
FEBS Lett. 1984 Jun 4;171(1):85-8. doi: 10.1016/0014-5793(84)80464-0.
8
Study of chaperone-like activity of human haptoglobin: conformational changes under heat shock conditions and localization of interaction sites.人触珠蛋白伴侣样活性的研究：热休克条件下的构象变化及相互作用位点的定位
Biol Chem. 2002 Oct;383(10):1667-76. doi: 10.1515/BC.2002.187.
9
Di-, tri-, and tetrapeptide sequences in haptoglobin. Contribution to understanding of haptoglobin structure.触珠蛋白中的二肽、三肽和四肽序列。对理解触珠蛋白结构的贡献。
Acta Biochim Pol. 1982;29(3-4):299-309.
10
A unique loop extension in the serine protease domain of haptoglobin is essential for CD163 recognition of the haptoglobin-hemoglobin complex.触珠蛋白丝氨酸蛋白酶结构域中独特的环延伸对于CD163识别触珠蛋白-血红蛋白复合物至关重要。
J Biol Chem. 2007 Jan 12;282(2):1072-9. doi: 10.1074/jbc.M605684200. Epub 2006 Nov 13.
引用本文的文献
1
Generation of a flexible loop structural ensemble and its application to induced-fit structural changes following ligand binding.柔性环结构集合体的生成及其在配体结合后诱导契合结构变化中的应用。
Biophysics (Nagoya-shi). 2006 Jan 31;2:1-12. doi: 10.2142/biophysics.2.1. eCollection 2006.
2
Biological function derived from predicted structures in CASP11.来自蛋白质结构预测技术关键评估第11轮（CASP11）中预测结构的生物学功能。
Proteins. 2016 Sep;84 Suppl 1(Suppl 1):370-91. doi: 10.1002/prot.24997. Epub 2016 Jun 15.
3
Assessment of template-based modeling of protein structure in CASP11.CASP11中基于模板的蛋白质结构建模评估。
Proteins. 2016 Sep;84 Suppl 1(Suppl 1):200-20. doi: 10.1002/prot.25049. Epub 2016 Jun 15.
4
Conformational sampling in template-free protein loop structure modeling: an overview.无模板蛋白质环结构建模中的构象采样：综述
Comput Struct Biotechnol J. 2013 Feb 25;5:e201302003. doi: 10.5936/csbj.201302003. eCollection 2013.
5
LEAP: highly accurate prediction of protein loop conformations by integrating coarse-grained sampling and optimized energy scores with all-atom refinement of backbone and side chains.LEAP：通过整合粗粒采样和优化能量评分，并结合主链和侧链的全原子精修，实现对蛋白质环构象的高精度预测。
J Comput Chem. 2014 Feb 5;35(4):335-41. doi: 10.1002/jcc.23509. Epub 2013 Dec 10.
6
CD163 binding to haptoglobin-hemoglobin complexes involves a dual-point electrostatic receptor-ligand pairing.CD163 通过与结合珠蛋白-血红蛋白复合物的双重静电受体-配体配对结合。
J Biol Chem. 2013 Jun 28;288(26):18834-41. doi: 10.1074/jbc.M113.471060. Epub 2013 May 13.
7
Defining and predicting structurally conserved regions in protein superfamilies.定义和预测蛋白质超家族中的结构保守区域。
Bioinformatics. 2013 Jan 15;29(2):175-81. doi: 10.1093/bioinformatics/bts682. Epub 2012 Nov 28.
8
New lives for old: evolution of pseudoenzyme function illustrated by iRhoms.旧酶新用：iRhoms 展示的伪酶功能进化
Nat Rev Mol Cell Biol. 2012 Jul 11;13(8):489-98. doi: 10.1038/nrm3392.
9
Identification of local conformational similarity in structurally variable regions of homologous proteins using protein blocks.利用蛋白质模块识别同源蛋白结构可变区的局部构象相似性。
PLoS One. 2011 Mar 18;6(3):e17826. doi: 10.1371/journal.pone.0017826.
10
Homology modelling and spectroscopy, a never-ending love story.同源建模与光谱学：一段永无止境的爱恋故事。
Eur Biophys J. 2010 Mar;39(4):551-63. doi: 10.1007/s00249-009-0531-0. Epub 2009 Aug 29.
本文引用的文献
1
Covalent structure of human haptoglobin: a serine protease homolog.人触珠蛋白的共价结构：一种丝氨酸蛋白酶同源物。
Proc Natl Acad Sci U S A. 1980 Jun;77(6):3388-92. doi: 10.1073/pnas.77.6.3388.
2
A possible three-dimensional structure of bovine alpha-lactalbumin based on that of hen's egg-white lysozyme.基于鸡蛋清溶菌酶的牛α-乳白蛋白可能的三维结构。
J Mol Biol. 1969 May 28;42(1):65-86. doi: 10.1016/0022-2836(69)90487-2.
3
The interpretation of protein structures: estimation of static accessibility.蛋白质结构的解读：静态可及性的评估
J Mol Biol. 1971 Feb 14;55(3):379-400. doi: 10.1016/0022-2836(71)90324-x.
4
The binding of hemoglobin to haptoglobin and its relation to subunit dissociation of hemoglobin.血红蛋白与触珠蛋白的结合及其与血红蛋白亚基解离的关系。
J Biol Chem. 1971 Jan 10;246(1):69-73.
5
Stereochemistry of cooperative effects in haemoglobin.血红蛋白协同效应的立体化学
Nature. 1970 Nov 21;228(5273):726-39. doi: 10.1038/228726a0.
6
Three-dimensional structure of tosyl-elastase.甲苯磺酰弹性蛋白酶的三维结构
Nature. 1970 Feb 28;225(5235):811-6. doi: 10.1038/225811a0.
7
Structure of crystalline -chymotrypsin. V. The atomic structure of tosyl- -chymotrypsin at 2 A resolution.结晶胰凝乳蛋白酶的结构。V. 对甲苯磺酰基-胰凝乳蛋白酶在2埃分辨率下的原子结构
J Mol Biol. 1972 Jul 21;68(2):187-240. doi: 10.1016/0022-2836(72)90210-0.
8
Structural similarities between alpha-lytic protease of Myxobacter 495 and elastase.粘细菌495的α-溶胞蛋白酶与弹性蛋白酶之间的结构相似性。
Nat New Biol. 1971 Feb 17;229(7):202-5. doi: 10.1038/newbio229202a0.
9
Immunological and biochemical investigations of human serum haptoglobin: composition of haptoglobin-haemoglobin intermediate, haemoglobin-binding sites and presence of additional alleles for beta-chain.人血清触珠蛋白的免疫学和生物化学研究：触珠蛋白-血红蛋白中间体的组成、血红蛋白结合位点及β链额外等位基因的存在
Nature. 1965 Sep 18;207(5003):1264-7. doi: 10.1038/2071264a0.
10
The structure of bovine trypsin: electron density maps of the inhibited enzyme at 5 Angstrom and at 2-7 Angstron resolution.牛胰蛋白酶的结构：5埃及2 - 7埃分辨率下被抑制酶的电子密度图。
J Mol Biol. 1974 Feb 25;83(2):185-208. doi: 10.1016/0022-2836(74)90387-8.
Zotero 插件