用固相免疫吸附剂对RNA肿瘤病毒的RNA依赖性DNA聚合酶进行亲和层析。
Affinity chromatography of RNA-dependent DNA polymerase from RNA tumor viruses on a solid phase immunoadsorbent.
作者信息
Livingston D M, Scolnick E M, Parks W P, Todaro G T
出版信息
Proc Natl Acad Sci U S A. 1972 Feb;69(2):393-7. doi: 10.1073/pnas.69.2.393.
Abstract
A solid phase immunoadsorbent specific for RNA-dependent DNA polymerase from murine and feline RNA tumor viruses has been prepared. The enzymes from murine and feline virus but not from avian virus bind to columns of this material. Bound enzymes can be eluted in active form. This method has permitted selective purification of viral enzyme from crude extracts of virus-transformed cells, since the immunoadsorbent has no affinity for cellular DNA polymerases.
摘要
已经制备了一种对来自鼠类和猫科动物RNA肿瘤病毒的RNA依赖性DNA聚合酶具有特异性的固相免疫吸附剂。来自鼠类和猫科动物病毒的酶能与这种材料的柱体结合，而来自禽类病毒的酶则不能。结合的酶可以以活性形式洗脱下来。由于这种免疫吸附剂对细胞DNA聚合酶没有亲和力，所以该方法能够从病毒转化细胞的粗提物中选择性地纯化病毒酶。
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