胎鼠颅骨成骨样细胞的特性研究

Characterization of osteoblast-like cells from fetal rat calvaria.

作者信息

Kadis B, Goodson J M, Offenbacher S, Bruns J W, Seibert S

出版信息

J Dent Res. 1980 Nov;59(11):2006-13. doi: 10.1177/00220345800590111901.

DOI:10.1177/00220345800590111901

Abstract

An improved method is described for the isolation of osteoblast-like cells from fetal rat calvaria. The cells are mononuclear, 8.3 mu in diameter, alkaline phosphate positive, and possess rough endoplasmic reticulum, mitochondria, Golgi, and cytoplasmic processes. Isolated bone cells synthesize collagen and cholesterol, grow to confluence in primary culture, and respond to hormonal stimulation.

摘要

描述了一种从胎鼠颅骨分离成骨细胞样细胞的改良方法。这些细胞为单核细胞，直径8.3微米，碱性磷酸酶呈阳性，具有粗面内质网、线粒体、高尔基体和细胞质突起。分离出的骨细胞能合成胶原蛋白和胆固醇，在原代培养中生长至汇合，并对激素刺激产生反应。