Albanese Paola, Mavelli Fabio, Altamura Emiliano
Department of Earth, Environmental and Physical Sciences, University of Siena, Siena, Italy.
Department of Biotechnology, Chemistry and Pharmaceutical Sciences, University of Siena, Siena, Italy.
Front Bioeng Biotechnol. 2023 Mar 29;11:1161730. doi: 10.3389/fbioe.2023.1161730. eCollection 2023.
In this work we review the latest strategies for the bottom-up assembly of energetically autonomous artificial cells capable of transducing light energy into chemical energy and support internalized metabolic pathways. Such entities are built by taking inspiration from the photosynthetic machineries found in nature which are purified and reconstituted directly in the membrane of artificial compartments or encapsulated in form of organelle-like structures. Specifically, we report and discuss recent examples based on liposome-technology and multi-compartment (nested) architectures pointing out the importance of this matter for the artificial cell synthesis research field and some limitations and perspectives of the bottom-up approach.
在这项工作中，我们回顾了自下而上组装能量自主人工细胞的最新策略，这些人工细胞能够将光能转化为化学能并支持内化的代谢途径。此类实体的构建灵感来源于自然界中发现的光合机制，这些光合机制被直接纯化并重构于人工隔室的膜中，或以细胞器样结构的形式被封装。具体而言，我们报告并讨论了基于脂质体技术和多隔室（嵌套）架构的近期实例，指出了这一问题对人工细胞合成研究领域的重要性以及自下而上方法的一些局限性和前景。
