对一个可逆控制元件的核苷酸序列的分析。
Analysis of the nucleotide sequence of an invertible controlling element.
作者信息
Zieg J, Simon M
出版信息
Proc Natl Acad Sci U S A. 1980 Jul;77(7):4196-200. doi: 10.1073/pnas.77.7.4196.
Abstract
The nucleotide sequence of the inversion region responsible for flagellar phase variation in Salmonella was determined. The inversion region is 995 base pairs (bp) in length and is bounded by a 14-bp inverted repeat sequence. A homologous recombination event between the 14-bp inverted repeat sequences would result in the inversion of the DNA segment between them. Sequence homologies with other systems suggest that the 14-bp inverted repeat sequences may have some general significance as sites for specific recombinational events. The gene which specifies H2 flagellin synthesis begins 16 bp outsie the inversion region. Within the inversion region, an open translational frame exists which could encode a low molecualr weight polypeptide (190 amino acids).
摘要
确定了沙门氏菌中负责鞭毛相变的倒位区域的核苷酸序列。该倒位区域长度为995个碱基对（bp），由一个14 bp的反向重复序列界定。14 bp反向重复序列之间的同源重组事件将导致它们之间的DNA片段发生倒位。与其他系统的序列同源性表明，14 bp反向重复序列作为特定重组事件的位点可能具有一些普遍意义。指定H2鞭毛蛋白合成的基因在倒位区域外16 bp处开始。在倒位区域内，存在一个开放的翻译框架，它可以编码一种低分子量多肽（190个氨基酸）。
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