扫描量热法证明磷脂酰胆碱双层膜中存在第三次相变。
Scanning calorimetric evidence for a third phase transition in phosphatidylcholine bilayers.
作者信息
Chen S C, Sturtevant J M, Gaffney B J
出版信息
Proc Natl Acad Sci U S A. 1980 Sep;77(9):5060-3. doi: 10.1073/pnas.77.9.5060.
Abstract
A third phase transition, centered at about 18 degrees C, was observed by differential scanning calorimetry in a multilamellar suspension of dipalmitoyl phosphatidylcholine that had been held at 0 degrees C for several days. This transition is less cooperative than the other two transitions which are well known for this system, and it is accompanied by an enthalpy increase about three times as large as that of the so-called pretransition at 35 degrees C and about half that of the main gel to liquid crystal transition at 41 degrees C. The reversal of this transition on cooling is extremely slow.
摘要
通过差示扫描量热法在已在0℃保持数天的二棕榈酰磷脂酰胆碱多层悬浮液中观察到了第三个相变，其中心温度约为18℃。该相变的协同性低于该体系中另外两个已知的相变，并且伴随着焓的增加，其增加量约为35℃时所谓预相变焓增加量的三倍，约为41℃时主要的凝胶到液晶相变焓增加量的一半。冷却时该相变的逆转极其缓慢。
相似文献
1
Scanning calorimetric evidence for a third phase transition in phosphatidylcholine bilayers.扫描量热法证明磷脂酰胆碱双层膜中存在第三次相变。
Proc Natl Acad Sci U S A. 1980 Sep;77(9):5060-3. doi: 10.1073/pnas.77.9.5060.
2
Thermodynamic characterization of the pretransition of unilamellar dipalmitoyl-phosphatidylcholine vesicles.单层二棕榈酰磷脂酰胆碱囊泡预转变的热力学表征。
Lipids. 1984 Jun;19(6):395-400. doi: 10.1007/BF02537400.
3
Fluorescence and calorimetric studies of phase transitions in phosphatidylcholine multilayers: kinetics of the pretransition.磷脂酰胆碱多层膜相变的荧光和量热研究：预转变动力学
Biochemistry. 1978 Oct 17;17(21):4475-80. doi: 10.1021/bi00614a018.
4
Characterization of a third phase transition in multilamellar dipalmitoyllecithin liposomes.多层二棕榈酰卵磷脂脂质体中第三种相变的表征
Biochemistry. 1981 Sep 29;20(20):5707-10. doi: 10.1021/bi00523a011.
5
Calorimetry of apolipoprotein-A1 binding to phosphatidylcholine-triolein-cholesterol emulsions.载脂蛋白A1与磷脂酰胆碱-三油酸甘油酯-胆固醇乳剂结合的量热法。
Biophys J. 1996 Jan;70(1):330-8. doi: 10.1016/S0006-3495(96)79574-7.
6
Properties of ganglioside GM1 in phosphatidylcholine bilayer membranes.神经节苷脂GM1在磷脂酰胆碱双层膜中的特性。
Biophys J. 1996 Mar;70(3):1363-72. doi: 10.1016/S0006-3495(96)79694-7.
7
Structure and thermotropic properties of 1-stearoyl-2-acetyl-phosphatidylcholine bilayer membranes.1-硬脂酰-2-乙酰磷脂酰胆碱双层膜的结构与热致性质
Biophys J. 1994 May;66(5):1469-78. doi: 10.1016/S0006-3495(94)80937-3.
8
Differential scanning calorimetric and Fourier transform infrared spectroscopic studies of the effects of cholesterol on the thermotropic phase behavior and organization of a homologous series of linear saturated phosphatidylserine bilayer membranes.差示扫描量热法和傅里叶变换红外光谱法研究胆固醇对一系列同系线性饱和磷脂酰丝氨酸双层膜的热致相行为和结构的影响。
Biophys J. 2000 Oct;79(4):2056-65. doi: 10.1016/S0006-3495(00)76453-8.
9
Dimyristoylphosphatidylcholine/C16:0-ceramide binary liposomes studied by differential scanning calorimetry and wide- and small-angle x-ray scattering.通过差示扫描量热法以及广角和小角X射线散射研究的二肉豆蔻酰磷脂酰胆碱/C16:0-神经酰胺二元脂质体
Biophys J. 2000 May;78(5):2459-69. doi: 10.1016/S0006-3495(00)76790-7.
10
Calorimetric and x-ray diffraction studies of rye glucocerebroside mesomorphism.黑麦葡萄糖脑苷脂介晶性的量热法和X射线衍射研究。
Biophys J. 1992 May;61(5):1289-300. doi: 10.1016/S0006-3495(92)81937-9.
引用本文的文献
1
Crystallization of -Alkanes under Anisotropic Nanoconfinement in Lipid Bilayers.脂质双层中各向异性纳米限域下正构烷烃的结晶
J Phys Chem B. 2025 Jan 9;129(1):435-446. doi: 10.1021/acs.jpcb.4c04332. Epub 2024 Dec 18.
2
Polymer-Lipid Hybrid Materials.聚合物-脂质杂化材料
Chem Rev. 2021 Nov 24;121(22):13996-14030. doi: 10.1021/acs.chemrev.1c00755. Epub 2021 Nov 9.
3
Preparation of DPPC liposomes using probe-tip sonication: Investigating intrinsic factors affecting temperature phase transitions.使用探头超声法制备二棕榈酰磷脂酰胆碱脂质体：探究影响温度相变的内在因素。
Biochem Biophys Rep. 2020 Apr 20;22:100764. doi: 10.1016/j.bbrep.2020.100764. eCollection 2020 Jul.
4
Conformational distributions and hydrogen bonding in gel and frozen lipid bilayers: a high frequency spin-label ESR study.凝胶和冷冻脂质双层中的构象分布和氢键：高频自旋标记 ESR 研究。
J Phys Chem B. 2012 Jun 14;116(23):6694-706. doi: 10.1021/jp211879s. Epub 2012 Mar 2.
5
A simple thermodynamic model of the liquid-ordered state and the interactions between phospholipids and cholesterol.一个简单的液体有序状态热力学模型以及磷脂和胆固醇之间的相互作用。
Biophys J. 2011 Jan 19;100(2):420-9. doi: 10.1016/j.bpj.2010.12.3694.
6
Combined use of steady-state fluorescence emission and anisotropy of merocyanine 540 to distinguish crystalline, gel, ripple, and liquid crystalline phases in dipalmitoylphosphatidylcholine bilayers.联合使用部花青540的稳态荧光发射和各向异性来区分二棕榈酰磷脂酰胆碱双层中的晶相、凝胶相、波纹相和液晶相。
PMC Biophys. 2010 Nov 5;3(1):14. doi: 10.1186/1757-5036-3-14.
7
Kinetics and dynamics of annealing during sub-gel phase formation in phospholipid bilayers: A saturation transfer electron spin resonance study.磷脂双层亚凝胶相形成过程中退火的动力学和动态学：饱和转移电子自旋共振研究。
Biophys J. 1993 Jun;64(6):1781-8. doi: 10.1016/S0006-3495(93)81549-2.
8
Time-resolved x-ray diffraction and calorimetric studies at low scan rates: I. Fully hydrated dipalmitoylphosphatidylcholine (DPPC) and DPPC/water/ethanol phases.时间分辨 X 射线衍射和低扫描速率下的量热研究：I. 完全水合的二棕榈酰磷脂酰胆碱（DPPC）和 DPPC/水/乙醇相。
Biophys J. 1989 Oct;56(4):757-68. doi: 10.1016/S0006-3495(89)82723-7.
9
Pressure locking of the subgel phase of hydrated dipalmitoyl phosphatidylcholine bilayers: a Raman spectroscopic study.水合二棕榈酰磷脂酰胆碱双层亚凝胶相的压力锁定：拉曼光谱研究。
Biophys J. 1986 Jun;49(6):1199-203. doi: 10.1016/S0006-3495(86)83748-1.
10
Structure of lipid tubules formed from a polymerizable lecithin.可聚合的磷脂形成的脂质小管的结构。
Biophys J. 1985 Dec;48(6):899-906. doi: 10.1016/S0006-3495(85)83852-2.
本文引用的文献
1
Kinetics of the crystalline-liquid crystalline phase transition of dimyristoyl L-alpha-lecithin bilayers.二肉豆蔻酰基-L-α-卵磷脂双层膜的晶态-液晶态相变动力学
Proc Natl Acad Sci U S A. 1974 Jul;71(7):2684-8. doi: 10.1073/pnas.71.7.2684.
2
Investigation of phase transitions of lipids and lipid mixtures by sensitivity differential scanning calorimetry.利用灵敏度差示扫描量热法研究脂质及脂质混合物的相变
Proc Natl Acad Sci U S A. 1976 Nov;73(11):3862-6. doi: 10.1073/pnas.73.11.3862.
3
Fluorescence and calorimetric studies of phase transitions in phosphatidylcholine multilayers: kinetics of the pretransition.磷脂酰胆碱多层膜相变的荧光和量热研究：预转变动力学
Biochemistry. 1978 Oct 17;17(21):4475-80. doi: 10.1021/bi00614a018.
4
Nature of the gel to liquid crystal transition of synthetic phosphatidylcholines.合成磷脂酰胆碱的凝胶态向液晶态转变的性质。
Proc Natl Acad Sci U S A. 1978 May;75(5):2258-60. doi: 10.1073/pnas.75.5.2258.
Zotero 插件