Ronneberger H, Zwisler O
Arch Toxicol Suppl. 1980;4:179-83. doi: 10.1007/978-3-642-67729-8_40.
Levine produced encephalitis in Lewis rats after injection of pertussis vaccine together with spinal cord tissue of guinea-pigs. This animal model was used as an assay for the possible side-effects of pertussis vaccines prepared from whole bacteria or with extracted antigens. Wistar and Lewis rats were injected with a mixture of guinea-pig spinal cord and cFA together with vaccines into the pad of each hind food. During a period of 25 days, the rats were observed for paralysis, ataxia, and death. Wistar rats were not found to be sensitive enough. Lewis rats were high susceptible in this model; they developed a high rate of allergic encephalopathy. DPT-vaccines prepared with soluble antigens showed a reduced neurotoxic activity. The results were compared with the histamine-sensitizing assay and the mouse weight-gain test. In these assays similar results were found. The proposed animal assay is recommended in the preclinical testing of pertussis vaccines.
莱文在给刘易斯大鼠注射百日咳疫苗以及豚鼠脊髓组织后使其患上了脑炎。该动物模型被用作一种检测方法，以评估由全细菌制备或使用提取抗原制备的百日咳疫苗可能产生的副作用。将豚鼠脊髓和完全弗氏佐剂的混合物与疫苗一起注射到Wistar大鼠和刘易斯大鼠每只后足的脚垫中。在25天的时间里，观察大鼠是否出现瘫痪、共济失调和死亡情况。发现Wistar大鼠不够敏感。刘易斯大鼠在该模型中高度易感；它们出现了高比例的过敏性脑病。用可溶性抗原制备的百白破疫苗显示出降低的神经毒性活性。将结果与组胺致敏试验和小鼠体重增加试验进行了比较。在这些试验中发现了相似的结果。建议在百日咳疫苗的临床前试验中采用所提出的动物检测方法。
