Ronneberger H, Blackkolb F, Guthöhrlein G
Arch Toxicol Suppl. 1982;5:129-32.
From the same batch of B. pertussis bacteria two types of DPT-vaccines were produced after harvest of the inactivated organisms by centrifugation or acid precipitation. The first vaccine contained whole pertussis bacteria and the second an extracted antigen complex. In a screening programme with reliable animal tests, such as a modified mouse-weight-gain assay, mouse leukocytosis test, histamine sensitization in mice, allergic encephalitis in rats, and the limulus test it could be demonstrated that the vaccine with whole inactivated bacteria was more toxic than that with extracted antigens. Cell material harvested by centrifugation showed a lower rate of toxicity than that containing acid-precipitated pertussis organisms.
从同一批百日咳杆菌中，通过离心或酸沉淀收获灭活生物体后，制备了两种类型的白百破疫苗。第一种疫苗含有完整的百日咳杆菌，第二种含有提取的抗原复合物。在一项采用可靠动物试验的筛选计划中，如改良的小鼠体重增加试验、小鼠白细胞增多试验、小鼠组胺致敏试验、大鼠过敏性脑炎试验和鲎试剂试验，可以证明含有完整灭活细菌的疫苗比含有提取抗原的疫苗毒性更大。通过离心收获的细胞物质的毒性率低于含有酸沉淀百日咳生物体的细胞物质。
