粪便胆汁酸的酶法测定。胆汁酸吸收不良的简易检测方法。

Enzymatic determination of faecal bile acids. A simple test for bile acid malabsorption.

作者信息

Ferraris R, Carosso R, Fiorentini M T, De La Pierre M, Marcellino M, Eandi M, Ricci Gamalero S

出版信息

Boll Soc Ital Biol Sper. 1980 Jan 15;56(1):15-21.

Abstract

A simple method for total faecal bile acid (FBA) determination was carried out. This test involves a 72 hour stool collection, homogenization and FBA determination by means of an enzymatic-fluorimetric method (3 -Hydroxysteroid-dehydrogenase). The reliability of this test was discussed. FBA levels were tested in twelve healthy volunteers. In addition, two patients with bile acid malabsorption syndrome due to terminal ileitis and one patient with cholegenic diarrhoea caused by biliocolonic fistula were examined. The mean +/- SD of FBA values in healthy subjects was 0.49 +/- 0.3 g/72 hours. However, all patients tested show a marked increase of FBA levels. In conclusion the present method for bile acid determination in the faeces seems to be a useful tool in the diagnosis of bile acid malabsorption syndrome and in cholegenic diarrhoea.

摘要

开展了一种测定总粪便胆汁酸（FBA）的简单方法。该检测包括72小时粪便收集、匀浆以及通过酶荧光法（3-羟基类固醇脱氢酶）测定FBA。讨论了该检测的可靠性。对12名健康志愿者的FBA水平进行了检测。此外，还检查了两名因末端回肠炎导致胆汁酸吸收不良综合征的患者以及一名因胆结肠瘘引起胆源性腹泻的患者。健康受试者FBA值的平均值±标准差为0.49±0.3g/72小时。然而，所有检测的患者FBA水平均显著升高。总之，目前粪便胆汁酸测定方法似乎是诊断胆汁酸吸收不良综合征和胆源性腹泻的有用工具。