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重复序列频率图谱分析：对包含人类起始甲硫氨酸转运RNA基因的克隆DNA片段内重复序列组织的分析。

Reiteration frequency mapping: analysis of repetitive sequence organization within cloned DNA fragments containing the human initiator methionine tRNA gene.

作者信息

Zasloff M, Santos T

出版信息

Proc Natl Acad Sci U S A. 1980 Oct;77(10):5668-72. doi: 10.1073/pnas.77.10.5668.

DOI:10.1073/pnas.77.10.5668
PMID:6934502
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC350130/
Abstract

The organization of repetitive sequences within three cloned chromosomal segments from human fetal liver DNA containing the initiator methionine tRNA gene was studied. The procedure developed for this study involves the contact hybridization of an electrophoretically separated 5'-32P-labeled restriction endonuclease digest of the cloned segment with total human genomic DNA covalently bound to aminobenzyloxymethyl-paper. The extent of hybridization of each labeled fragment to the area of paper with which it is in contact is proportional to the representation of the sequence within the human genome. We show that sequences with a wide range of genomic repetitition are present in the neighborhoods of the three dispersed initiator tRNA loci, each characterized by a different overall organization pattern.

摘要

对来自人胎儿肝脏DNA的三个克隆染色体片段中重复序列的组织进行了研究，这些片段包含起始甲硫氨酸tRNA基因。本研究开发的方法涉及将克隆片段经电泳分离的5'-32P标记限制性内切酶消化产物与共价结合在氨基苄氧基甲基纸上的总人基因组DNA进行接触杂交。每个标记片段与接触区域的杂交程度与该序列在人类基因组中的代表性成正比。我们发现，在三个分散的起始tRNA基因座附近存在具有广泛基因组重复的序列，每个基因座具有不同的整体组织模式。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cba/350130/f1b6bb83ba16/pnas00497-0132-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cba/350130/265d0789775f/pnas00497-0130-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cba/350130/a6ebb3eac98f/pnas00497-0131-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cba/350130/52523aeb57c1/pnas00497-0132-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cba/350130/0083d3a4b33c/pnas00497-0132-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cba/350130/f1b6bb83ba16/pnas00497-0132-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cba/350130/265d0789775f/pnas00497-0130-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cba/350130/a6ebb3eac98f/pnas00497-0131-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cba/350130/52523aeb57c1/pnas00497-0132-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cba/350130/0083d3a4b33c/pnas00497-0132-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cba/350130/f1b6bb83ba16/pnas00497-0132-c.jpg

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本文引用的文献

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