Blin N, Stafford D W
Nucleic Acids Res. 1976 Sep;3(9):2303-8. doi: 10.1093/nar/3.9.2303.
A new method for isolation of high molecular weight DNA from eukaryotes is presented. This procedure allows preparation of DNA from a variety of tissues such as calf thymus or human placenta and from cells which were more difficult to lyse until now (e.g. Crypthecodinium cuhnii, a dinoflagellate). The DNA obtained in such a way has an average molecular weight of about 200 X 10(6) d and contains very few, if any, single strand breaks.
本文介绍了一种从真核生物中分离高分子量DNA的新方法。该方法可从多种组织（如小牛胸腺或人胎盘）以及迄今较难裂解的细胞（如一种甲藻，隐甲藻）中制备DNA。以这种方式获得的DNA平均分子量约为200×10⁶ d，且几乎没有单链断裂（若有也极少）。
