一种从真核生物中分离高分子量DNA的通用方法。
A general method for isolation of high molecular weight DNA from eukaryotes.
作者信息
Blin N, Stafford D W
出版信息
Nucleic Acids Res. 1976 Sep;3(9):2303-8. doi: 10.1093/nar/3.9.2303.
Abstract
A new method for isolation of high molecular weight DNA from eukaryotes is presented. This procedure allows preparation of DNA from a variety of tissues such as calf thymus or human placenta and from cells which were more difficult to lyse until now (e.g. Crypthecodinium cuhnii, a dinoflagellate). The DNA obtained in such a way has an average molecular weight of about 200 X 10(6) d and contains very few, if any, single strand breaks.
摘要
本文介绍了一种从真核生物中分离高分子量DNA的新方法。该方法可从多种组织（如小牛胸腺或人胎盘）以及迄今较难裂解的细胞（如一种甲藻，隐甲藻）中制备DNA。以这种方式获得的DNA平均分子量约为200×10⁶ d，且几乎没有单链断裂（若有也极少）。
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本文引用的文献
1
SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA.DNA大小与形状的沉降研究
J Mol Biol. 1965 Feb;11:373-90. doi: 10.1016/s0022-2836(65)80064-x.
2
Sedimentation rate as a measure of molecular weight of DNA.沉降速率作为DNA分子量的一种衡量指标。
Biophys J. 1963 Jul;3(4):309-21. doi: 10.1016/s0006-3495(63)86823-x.
3
The nucleic acids of Drosophila melanogaster.黑腹果蝇的核酸。
Biochem J. 1966 Aug;100(2):532-9. doi: 10.1042/bj1000532.
4
Hydrodynamic shear breakage of DNA.DNA的流体动力剪切断裂
Biopolymers. 1972;11(12):2601-24. doi: 10.1002/bip.1972.360111217.
5
Sedimentation properties of yeast nuclear and mitochondrial DNA.酵母核DNA和线粒体DNA的沉降特性。
J Mol Biol. 1972 Jun 14;67(1):11-24. doi: 10.1016/0022-2836(72)90382-8.
6
Unique double-stranded fragments of bacteriophage T5 DNA resulting from preferential shear-induced breakage at nicks.噬菌体T5 DNA独特的双链片段，由切口处优先的剪切诱导断裂产生。
Proc Natl Acad Sci U S A. 1974 May;71(5):2108-12. doi: 10.1073/pnas.71.5.2108.
7
Isolation of high-molecular-weight DNA from mammalian cells.从哺乳动物细胞中分离高分子量DNA。
Eur J Biochem. 1973 Jul 2;36(1):32-8. doi: 10.1111/j.1432-1033.1973.tb02881.x.
8
Isolation of large molecular weight DNA from agarose gels for further digestion by restriction enzymes.从琼脂糖凝胶中分离大分子量DNA以便用限制性内切酶进一步消化。
FEBS Lett. 1975 Apr 15;53(1):84-6. doi: 10.1016/0014-5793(75)80688-0.
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