Naylor S L, Sakaguchi A Y, Shows T B, Grzeschik K H, Holmes M, Zasloff M
Proc Natl Acad Sci U S A. 1983 Aug;80(16):5027-31. doi: 10.1073/pnas.80.16.5027.
Two nonallelic human tRNAiMet genes were assigned to chromosome 6 by filter hybridization of DNA from human-rodent somatic cell hybrids by using probes containing unique sequences from the regions flanking each tRNAiMet gene. These unique sequence probes thus allowed each tRNAiMet gene to be analyzed individually in cell hybrids. Both tRNAiMet genes segregated in the hybrid cells with the chromosome 6 enzyme markers, soluble malic enzyme and the mitochondrial form of superoxide dismutase, and also with a karyotypically normal chromosome 6. By using hybrid clones containing translocations that divide chromosome 6 into five segments, both tRNAiMet genes were assigned to the p23 leads to q12 region. These results raise the possibility that other tRNAiMet genes may be syntenic with the two described in this study and illustrate the utility of using unique flanking sequences to identify members of a multigene family.
通过使用包含每个甲硫氨酸起始转运RNA（tRNAiMet）基因侧翼区域独特序列的探针，对人-啮齿动物体细胞杂种的DNA进行滤膜杂交，将两个非等位的人类tRNAiMet基因定位到了6号染色体上。因此，这些独特序列探针使得每个tRNAiMet基因能够在细胞杂种中单独进行分析。两个tRNAiMet基因在杂种细胞中与6号染色体的酶标记物——可溶性苹果酸酶和线粒体形式的超氧化物歧化酶，以及一条核型正常的6号染色体一起分离。通过使用含有将6号染色体分成五个区段的易位的杂种克隆，两个tRNAiMet基因都被定位到了p23至q12区域。这些结果增加了其他tRNAiMet基因可能与本研究中描述的两个基因同线的可能性，并说明了使用独特侧翼序列来鉴定多基因家族成员的实用性。
