Raina J L, Ravin A W
Proc Natl Acad Sci U S A. 1980 Oct;77(10):6062-6. doi: 10.1073/pnas.77.10.6062.
The induction of synchronous development of competence for genetic transformation in Streptococcus sanguis, by either endogenous or exogenous competence factor (CF), is manifested in the transient synthesis of a new set of at least 10 polypeptides, ranging from 14,000 to 51,000 in molecular weight. Eight polypeptides (E14, E16, E24, E28, E32, E37, E44, E51) appear early, and two polypeptides (L34, L42) appear 5-10 min later. One of the newly synthesized early polypeptides, E16, is shown to be a component of the presynaptic complex containing single-stranded DNA that is produced in vivo upon uptake of native donor DNA. Concomitant with this induced synthesis of competence-specific polypeptides there is a net decrease in RNA and protein synthesis but no change in DNA synthesis; donor DNA-binding ability and transformability reach maxima during the phase of diminishing macromolecular synthesis. Subsequently, donor DNA-binding ability and transformability decay at disproportionate rates as cells return to the normal state of macromolecular synthesis within one generation. Coincident with the induction of competence, the synthesis of a new RNA transcript of high molecular weight appears to be induced which continues during the restricted phase of total cellular RNA synthesis.
通过内源性或外源性感受态因子（CF）诱导血链球菌中遗传转化感受态的同步发育，表现为一组新的至少10种多肽的瞬时合成，其分子量在14,000至51,000之间。八种多肽（E14、E16、E24、E28、E32、E37、E44、E51）出现较早，两种多肽（L34、L42）在5-10分钟后出现。新合成的早期多肽之一E16被证明是突触前复合物的一个组成部分，该复合物包含在摄取天然供体DNA后体内产生的单链DNA。伴随着这种感受态特异性多肽的诱导合成，RNA和蛋白质合成出现净减少，但DNA合成没有变化；在大分子合成减少阶段，供体DNA结合能力和转化能力达到最大值。随后，随着细胞在一代内恢复到大分子合成的正常状态，供体DNA结合能力和转化能力以不成比例的速率下降。与感受态的诱导同时，一种新的高分子量RNA转录本的合成似乎被诱导，并且在总细胞RNA合成的受限阶段持续进行。
