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C 群链球菌噬菌体溶素的纯化

Purification of Streptococcus group C bacteriophage lysin.

作者信息

Raina J L

出版信息

J Bacteriol. 1981 Jan;145(1):661-3. doi: 10.1128/jb.145.1.661-663.1981.

DOI:10.1128/jb.145.1.661-663.1981
PMID:7462154
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC217323/
Abstract

A simple procedure for the purification of Streptococcus group C phage lysin to apparent homogeneity is described. The electrophoretically pure, enzymatically stable polypeptide of 98,000 molecular weight converted Streptococcus (groups A, F, and H) cells into spheroplasts within 5 min at 0 degrees C or within less than a minute at 37 degrees C.

摘要

描述了一种将C组链球菌噬菌体溶素纯化至明显均一性的简单方法。这种电泳纯、酶稳定的98,000分子量多肽，在0℃下5分钟内或37℃下不到1分钟内就能将（A、F和H组）链球菌细胞转化为原生质球。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a231/217323/9a3535607155/jbacter00272-0683-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a231/217323/9a3535607155/jbacter00272-0683-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a231/217323/9a3535607155/jbacter00272-0683-a.jpg

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本文引用的文献

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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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Proc Natl Acad Sci U S A. 1980 Oct;77(10):6062-6. doi: 10.1073/pnas.77.10.6062.