Chang J J, Gelperin A
Proc Natl Acad Sci U S A. 1980 Oct;77(10):6204-6. doi: 10.1073/pnas.77.10.6204.
The isolated lips and nervous system of the terrestrial slug Limax maximus will produce some of the feeding behavior of the intact animal; i.e., they generate the rhythmic neural activity characteristic of ingestion in response to food extracts applied to the lips. This preparation will respond to a variety of food extracts that elicit feeding in the whole animal. This provides the opportunity for aversive conditioning experiments involving taste discrimination. Pairing lip chemostimulation by attractive food extracts with lip chemostimulation by using bitte plant secondary substances can cause the isolatd brain to selectively suppress its neural response to one food extract while remaining responsive to another. Such isolated brains can learn after one or two trials and retain the learning for more than 8 hr.
将陆地蛞蝓大蛞蝓分离出的唇部和神经系统会产生完整动物的一些进食行为；也就是说，它们会在将食物提取物施加到唇部时，产生摄入食物时特有的节律性神经活动。这种制备物会对多种能在完整动物中引发进食行为的食物提取物做出反应。这为涉及味觉辨别力的厌恶条件反射实验提供了机会。将有吸引力的食物提取物引起的唇部化学刺激与使用苦味植物次生物质引起的唇部化学刺激配对，可以使分离出的大脑选择性地抑制其对一种食物提取物的神经反应，同时保持对另一种食物提取物的反应能力。这样分离出的大脑在经过一两次试验后就能学习，并能将学习成果保持8小时以上。
